摘要
目的探讨三七总皂苷(PNS)预处理对缺血再灌注损伤肾脏wnt4蛋白的影响。方法取72只SD雄性大鼠随机分为对照(CON)组、缺血再灌注损伤(IR)组、PNS组。实验干预:(1)CON组切除右肾后,左肾暴露30 min后关腹。(2)IR组采用右肾切除,左侧肾动脉夹闭30 min恢复血流方法造模。(3)PNS组实验前尾静脉注射PNS(150mg/kg),共3 d,其余处理同IR组。在缺血再灌注后24 h、48 h、72 h,每个时间点每组处死8只大鼠,取血清检测肌酐(Scr)、尿素氮(BUN),留取左肾行HE染色,光镜下观察病理变化并进行肾小管损伤评分,免疫组化检测wnt4在肾组织内的表达情况,蛋白印记检测各组wnt4表达量。结果 IR组可见明显的肾脏病理损伤,肾小管损伤评分、Scr、BUN在24 h达到损伤顶峰,此后逐渐下降,72 h仍高于CON组(P<0.05),而PNS组各时间点肾小管损伤评分、Scr、BUN均较IR组降低(P<0.05)。wnt4的免疫组化及蛋白印迹结果一致,即IR组、PNS组wnt4表达量在24 h达到高峰,但PNS组各时间点表达量均较IR组升高(P<0.05)。结论 PNS对缺血再灌注损伤的肾脏具有保护作用,其作用机制可能与其增强wnt4蛋白表达有关。
Objective To observe the effect of panax notoginseng saponins(PNS)pretreatment on the expression ofwnt4protein in renal ischemia reperfusion injury in rats.Methods Seventy-two male SD rats were randomly averaged intocontrol group(CON group),renal ischemia-reperfusion group(IR group)and PNS group.Rats in CON group were removedthe right kidneys,and the left kidneys were exposed for30minutes,and then the incisions were closed.Rats of IR groupwere removed the right kidneys,and the left renal artery were clamped for30minutes,and then the incisions were closed.Rats in PNS group were injected PNS(150mg/kg)via tail vein for3days before experiment,and the rest steps were the samewith IR group.After24h,48h and72h reperfusion,8rats in each group were killed,and blood and kidney samples werecollected to examine the serum creatinine(Scr)and blood urea nitrogen(BUN).The left renal tissues were stained with HE.Pathological changes were observed under light microscopy.The score of renal tubular injury was evaluated.Immunohistochemistry was performed to detecte the expression of wnt4in kidney tissue.The expression of wnt4was detectedby Western blot assay.Results There was significant renal injury in IR group.The renal tubular injury score,Scr and BUNreached the peak levels of injury at24h,which were then decreased gradually,but at72h still significantly higher thanthose of CON group(P<0.05).The renal tubular injury score,Scr and BUN values at different time points were significantlylower in PNS group than those of IR group(P<0.05).The results of wnt4immunohistochemistry and Western blot assaywere consistent,which showed that the expression of wnt4peaked at24h in IR group and PNS group,but the expression levels were significantly higher in PNS group than those of IR group at each time point(P<0.05).Conclusion PNS couldprotect kidney from ischemia-reperfusion injury,which may be related to the enhanced expression of wnt4protein.
作者
林辉
张祥贵
LIN Hui;ZHANG Xiang-gui(Department of Nephrology, the Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai 519000, China)
出处
《天津医药》
CAS
2017年第5期472-476,共5页
Tianjin Medical Journal
基金
贵州省科技厅、遵义医学院、遵义市科技局联合基金项目(黔科合J字LKZ[2013]32号)
