摘要
目的探讨胃腺癌及癌旁正常组织中生长分化因子15(growth differentiation factor-15,GDF-15)和p53表达的特征及临床意义。方法采用免疫组织化学Polink-1两步法检测91例胃癌手术标本,相应91例癌旁正常组织、64例肠化腺体及36例淋巴结转移癌组织中GDF-15和p53的表达特征,并结合临床资料分析其表达与临床病理参数的关系。结果 GDF-15在肠上皮化生腺体、胃腺癌和淋巴结转移癌组织中的阳性表达率均显著低于癌旁组织。GDF-15的阳性表达率与患者年龄、浸润深度、淋巴结转移及p TNM分期呈正相关,而与其它临床病理参数无关。p53在癌旁正常胃粘膜腺体和肠上皮化生腺体中均不表达,在胃腺癌和淋巴结转移癌组织中的过表达阳性率均高于癌旁组织和肠上皮化生腺体。p53过表达的阳性率与患者年龄、Lauren分型(肠型与弥漫型)和分化程度呈正相关,而与其它临床病理参数无关。GDF-15与p53免疫反应性在胃癌组织中呈正相关。结论 GDF-15可作为胃腺癌的胃壁浸润能力、淋巴结转移能力及临床病理分期的重要评价指标,而p53过表达可能与Lauren分型的肠型胃癌有关。尤其在老年患者中,GDF-15和p53过表达在胃腺癌的发生、进展中扮演重要角色,并起协同作用。
Objective To investigate the characteristics and clinical significance of the expression of growth differentiation factor15(GDF-15)and the overexpression of p53in gastric adenocarcinoma and adjacent normal tissue.Methods The expression of GDF-15and p53were detected by Polink-1two-step immunohistochemical method in91samples from gastric adenocarcinoma and adjacent normal tissue,also in64samples with intestinal metaplasia and36lymph node tissue with metastasis adenocarcinoma.The relationship between the expression of these proteins and clinicopathologic features was analyzed.Results The positive rate of GDF-15expression was obviously lower in intestinal metaplasia tissue,gastric adenocarcinoma tissue and lymph node metastasis adenocarcinoma tissue than that in normal gastric tissue.GDF-15expression was positively correlated with age,depth of tumor invasion,lymph node metastasis and pTNM staging,while not related to other clinicopathologic features.There was no p53expression in normal gastric tissue and intestinal metaplasia tissue.The positive rate of p53overexpression was significantly higher in gastric adenocarcinoma tissue and lymph node metastasis adenocarcinoma tissue than that in normal gastric tissue and intestinal metaplasia tissue.P53overexpression was positively correlated with age,Lauren’s type(intestinal pattern and diffused pattern)and differentiation degree,while not related to other clinicopathologic features.GDF-15expression and p53overexpression were positively correlated in gastric cancer tissue.Conclusion GDF-15is a potential index for assessing the ability of gastric wall invasion,lymph node metastasis and pTNM staging of gastric adenocarcinoma.P53overexpression may be related to the intestinal pattern of Lauren’s type of gastric adenocarcinoma.GDF-15expression and p53overexpression cooperatively play an important role in the occurrence and development of gastric cancer,especially among elder patients.
作者
王文军
何雷
张帆
李佳嘉
徐国祥
徐五琴
Wang Wenjun;He Lei;Zhang Fan;Li Jiajia;Xu Guoxiang;Xu Wuqin(Department of Pathology,Yijishan Hospital of Wannan Medical College,Wuhu 241000,China)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2017年第3期255-261,共7页
Chinese Journal of Histochemistry and Cytochemistry
基金
安徽省高校自然科学研究项目(KJ2011Z394)
