摘要
利用多巴胺在材料表面的自发黏附特性,制备了多巴胺改性的聚丙烯纤维膜,研究多巴胺改性对副干酪乳杆菌(Lactobacillus paracaise)在聚丙烯纤维膜上形成生物膜的影响及L.paracasei生物膜的发酵性能。利用扫描电子显微镜、红外光谱仪及接触角仪对多巴胺改性聚丙烯纤维膜表面形成的L.paracasei生物膜进行表征,并对L.paracasei生物膜的抗逆性与发酵能力进行研究。结果表明,多巴胺改性对聚丙烯纤维膜表面L.paracasei生物膜形成具有显著的促进作用,多巴胺改性聚丙烯纤维膜表面的生物膜具有极佳的抗逆性能,更耐受高温和酸碱环境,用于生物膜反应器中发酵生产的L-乳酸纯度维持在99%以上,同时反应器基本没有发酵延滞期,有效提高了生物发酵效率。
Since dopamine can self-polymerize and adhere to the material surface in mild aqueous environments,dopaminewas used for surface modification of polypropylene fiber membranes.In this paper,the effect of surface modification ofpolypropylene fiber membranes with dopamine on the formation of Lactobacillus paracasei biofilms was studied.TheL.paracasei biofilms were characterized by scanning electron microscope,attenuated total reflectance Fourier transforminfrared spectroscopy,and water contact angle measurement.The stress resistance and fermentation performance for lacticacid production were studied in detail.The results demonstrated that the dopamine layer on the surface of polypropylenefiber membranes remarkably enhanced the formation of L.paracasei biofilms.It is worth to note that as compared with theL.paracasei on polypropylene fiber membranes,L.paracasei biofilms on dopamine-modified polypropylene fibermembranes possessed higher resistance to severe environments,such as high temperature,strong acid and strong base.Besides,the purity of L-lactic acid produced by L.paracasei biofilms in a biofilm reactor was higher than99%.The lagphase of fermentation scarcely existed,which means that high fermentation efficiency.
作者
赵子舒
李吉年
郭谦
叶翔宇
胡梦欣
ZHAO Zishu;LI Jinian;GUO Qian;YE Xiangyu;HU Mengxin(School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China;Zhejiang Textile Testing and Research Institute, Hangzhou 310018, China;Zhejiang Provincial Key Laboratory of Food Microbial Technology, Hangzhou 310018, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第14期71-77,共7页
Food Science
基金
国家自然科学基金青年科学基金项目(21004051)
中国博士后科学基金面上资助项目(2015M580500)
浙江省博士后科研项目择优资助项目
浙江省教育厅科研项目(Y201018929)
浙江省新型吸附分离材料与应用技术重点实验室开放基金项目(XFFL2015)
浙江省食品科学与工程重中之重一级学科开放基金项目(JYTSP20142103)
