摘要
目的:观察丹参酮ⅡA(TanⅡA)对压力超负荷大鼠心肌骨桥蛋白表达的影响,探讨TanⅡA防治心肌纤维化的作用机制。方法:在50只雄性6周龄SD大鼠中随机取42只行腹主动脉缩窄术制备压力超负荷模型(手术组),另外8只大鼠行假手术(假手术组)。4周后,手术组大鼠死亡12只,假手术组大鼠无死亡。将存活的30只手术组大鼠随机分为模型组、TanⅡA高剂量组和TanⅡA低剂量组。给药4周后,处死所有大鼠,HE和Masson染色观察大鼠心肌病理学改变,测量胶原容积分数(CVF)和血管周围胶原容积分数(PCVF)。碱水法检测心肌羟脯氨酸含量。免疫组织化学法分析心肌α-平滑肌肌动蛋白(α-SMA)、结缔组织生长因子(CTGF)和骨桥蛋白的表达水平。结果:TanⅡA能显著减少压力超负荷大鼠心肌CVF、PCVF、羟脯氨酸含量,降低α-SMA、CTGF、骨桥蛋白的表达水平(P<0.05),且高剂量TanⅡA的改善作用更为明显。结论:TanⅡA改善压力超负荷大鼠心肌纤维化的作用机制可能与抑制骨桥蛋白的表达有关。
Objective:To study the mechanism of Tanshinone II A(TanⅡA)in the prevention and treatmen myocardial fibrosis by observing its effects on the expression of myocardial osteopontin in pressure overload rats.Methods:A total of42rats were randomly chosen from50male SD rats with six weeks in age to prepare pressure overloadmodels byabdominal aorticconstriction,andother eight ratswere performedshamoperation.Four weeks later,30survived rats in the operation group were randomly divided into the model group,high and low dosegroupsofTanⅡA.Aftermedication for four weeks,pathological changes ofmyocardiumin rats were observed by HE and Masson staining;collagen volume fraction(CVF)and perivascular collagen volume fraction(PCVF)were determined.The content of myocardial hydroxyproline was tested by alkali hydrdysis method.The expressions of琢-SMA,CTGFand osteopontinwere analyzed by immunohistochemical methods.Results:TanⅡAcould significantly reduce myocardial CVF,PCVF and the contents of myocardial hydroxyproline,and decrease the expressions of琢-SMA,CTGF and osteopontin in pressure overload rats(P<0.05),and the improvement of high dose TanⅡA was more obvious.Conclusion:The mechanism of TanⅡA in improving myocardial fibrosis in pressure overload rats might be related to inhibiting the expressions of myocardial osteopontin.
作者
张蓓蓓
张静
常文静
蔡辉
ZHANG Beibei;ZHANG Jing;CHANG Wenjing;CAI Hui(Department of Integrated Traditional Chinese and Western Medicine,Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China)
出处
《西部中医药》
2017年第6期17-21,共5页
Western Journal of Traditional Chinese Medicine
