摘要
目的:探讨miR-34a在食管癌上皮间质转化中的作用。方法:向食管癌细胞EC109转染miR-34a模拟物,以q RT-PCR检测效率。以Western blot检测EMT相关蛋白E-cadherin和Vimentin的表达。结果:伴随miR-34a表达升高,E-cadherin相应升高,Vimentin则下降(P<0.05)。结论:miR-34a可促进食管癌EMT的发生。
Objective:To investigate the role of miR-34a in epithelial mesenchymal transition of esophageal carcinoma.Methods:MiR-34a analog was transfected into esophageal cancer cell line EC109,and the efficiency of qRT-PCR detection was detected.Western and blot were used to detect the expression of EMT related proteins E-cadherin and Vimentin.Results:With the increase of miR-34a expression,E-cadherin increased and Vimentin decreased(P<0.05).Conclusion:MiR-34a can promote the occurrence of EMT in esophageal carcinoma.
作者
姬玉
朱建波
李梅
吴菲
杨兰
李锋
Ji Yu;Zhu Jianbo;Li Mei;Wu Fei;Yang Lan;Li Feng(Department of Pathology,Medical College f Shihezi University 832000;Department of Pathology,Beijing Chaoyang Hospital Affiliated to Capital Medical University 100016)
出处
《中国社区医师》
2017年第23期5-5,7,共2页
Chinese Community Doctors
基金
国家自然科学基金编号:81260301~~
