摘要
蜡样芽孢杆菌是一种食源性条件致病菌,在环境和食品中分布比较广泛,所引起的食物中毒常分为腹泻型和呕吐型。研究蜡样芽孢杆菌的分型方法对该菌的预防、预警、溯源及分子流行病学的调查都具有重要意义。目前,蜡样芽孢杆菌的分型方法主要包括噬菌体分型、生化分型等传统分型方法以及多位点序列分型、重复序列聚合酶链式反应分型、随机扩增多态性DNA分型、脉冲场凝胶电泳分型等分子分型方法。以蜡样芽孢杆菌为对象,综述其分型方法的研究进展,以期为该菌的分型提供一定参考。
Bacillus cereus is a food-borne opportunistic pathogen widely present in the environment and foods.It is frequently associated with two types of food-borne illness:emesis and diarrhea.The study on Bacillus cereus typing has important implications for the prevention,early warning,tracing and molecular epidemiological survey of Bacillus cereus.Currently,the popular methods for typing Bacillus cereus include traditional typing methods such as phage typing and biochemical typing,and molecular typing methods such as multilocus sequence typing,repetitive sequence-PCR,random amplified polymorphic DNA,and pulsed-field gel electrophoresis.In this paper,all these methods are reviewed with the aim of providing references for the typing of Bacillus cereus.
作者
曹飞扬
王娉
江连洲
陈颖
CAO Feiyang;WANG Ping;JIANG Lianzhou;CHEN Ying(Agro-Product Safety Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100176, China;College of Food Science, Northeast Agricultural University, Harbin 150030, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第17期286-290,共5页
Food Science
基金
"十三五"国家重点研发计划重点专项(2016YFD0401102)
中国检验检疫科学研究院院所基金项目(2016JK006)
关键词
蜡样芽孢杆菌
传统分型
分子分型
Bacillus cereus
traditional typing methods
molecular typing methods
