摘要
建立了测定豆芽中4-氯苯氧乙酸钠和6-苄基腺嘌呤的固相萃取–高效液相色谱法。样品以0.1 mol/L的盐酸溶液为提取剂,经C18固相萃取柱净化处理,以乙腈–10 mmol/L乙酸铵水溶液(体积比25∶75)为流动相,Tech Mate C_(18)–ST色谱柱分离,4-氯苯氧乙酸钠和6-苄基腺嘌呤的紫外检测波长分别为228 nm和267 nm,色谱峰面积外标法定量。在0.50~100.00 mg/L浓度范围内,4-氯苯氧乙酸钠的相关系数为0.999 8,精密度在1.3%~8.2%之间(n=6),回收率在103.1%~109.0%之间;在0.05~10 mg/L浓度范围内,6-苄基腺嘌呤相关系数为0.999 9,精密度在0.9%~3.8%之间(n=6),回收率在88.0%~95.4%之间。4-氯苯氧乙酸钠和6-苄基腺嘌呤的检出限(S/N=3)分别为0.24,0.02 mg/kg。该法可以同时完成豆芽中4-氯苯氧乙酸钠和6-苄基腺嘌呤的分析检测。
The SPE-HPLC method for determination of4-chlorobenzene sodium acetate and6-benzyladenine in bean sprout was established.Samples were extracted with0.1mol/L hydrochloric acid and purified by C18SPE column.The target compounds were separated by TechMate C18-ST column with acetonitrile-10mmol/L ammonium acetate solution(25∶75)as mobile phase.The UV detection wavelength of4-chlorobenzene sodium acetate and6-benzyladenine were228nm and267nm,respectively.The determinaion was performed by peak area with external standard method.The results showed that the linear range of4-chlorobenzene sodium acetate was0.5-100.00mg/L with the linear correlation coefficients of0.9998,RSD of determination results were1.3%-8.2%and the recoveries were ranged from103.1%to109.0%;the linear range of6-benzyladenine was0.05-10mg/L(n=6)with the linear correlation coefficients of0.9999,RSD of determination results were0.9%-3.8%(n=6)and the recoveries were ranged from88.0%to95.4%.The method detection limit of4-chlorobenzene sodium acetate and6-benzyladenine were0.24mg/kg and0.02mg/kg,respectively.The method can be used to analyze4-chlorobenzene sodium acetate and6-benzylaminopurin in bean sprouts at the same time.
作者
芦智远
冯歆轶
汪静雯
徐斐
杨琦
Lu Zhiyuan;Feng Xinyi;Wang Jingwen;Xu Fei;Yang Qi(Xi’an Product Quality Supervision and Inspection Institute, Xi’an 710065, China;College of Life Science and Food Engineering Shanxi Xueqian Normal University, Xi’an 710100, China)
出处
《化学分析计量》
CAS
2017年第5期55-58,共4页
Chemical Analysis And Meterage
关键词
豆芽
4-氯苯氧乙酸钠
6-苄基腺嘌呤
高效液相色谱法
固相萃取
bean sprout
4-chlorobenzene sodium acetate
6-benzylaminopurin
high performance liquid chromatography
solid phase extraction
