摘要
牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)有两种基因型:基因1型(BVDV-1)和基因2型(BVDV-2)。以往的流行病学调查显示BVDV-1的分布和流行区域更加广泛,但近年来国内BVDV-2的发病率呈持续升高趋势。BVDV-2通常会引起严重的急性感染和出血综合征,给养牛业造成巨大损失。本文就BVDV-2特异性检测方法和基因分型的建立,型特异性BVDV-2毒株的分离与鉴定,BVDV-2优势分离株的全基因组测定与遗传特性分析及BVDV-1和BVDV-2抗原和抗体检测平台的建立等方面展开简要综述。
Bovine viral diarrhea virus is divided into two genotypes:genotype1(BVDV-1)and genotype2(BVDV-2).Previous epidemiological survey showed BVDV-1isolates distributed more widely.However,the mobidity rate of BVDV-2is increasing in recent years.In general,BVDV-2infection can cause more severe disease characterized by acute infection and haemorrhagic syndrome,causing enormous losses to the cattle industry.In this review,we dilate on the following aspects:the establishment of the specifi c detection and genotyping methods of BVDV-2,the isolation and identifi cation of BVDV-2,complete genomic sequencing and phylogenetic analyzation of BVDV-2isolates,and the establishment of detection platform to virus and antibody of BVDV-1and BVDV-2.
作者
张倩
陶洁
张东
区炳明
林航
杨颖
张信军
朱礼倩
王建业
贺生中
孟婷
谢静
朱国强
ZHANG Qian;TAO Jie;ZHANG Dong;OU Bing-ming;LIN Hang;YANG Ying;ZHANG Xinjun;ZHU Li-qian;WANG Jian-ye;HE Sheng-zhong;MENG Ting;XIE Jing;ZHU Guo-qiang(College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;Prevention and Control of Collaborative Innovation Center of the Important Animal Disease and Zoonosis in Jiangsu Province, Yangzhou 225009, China;Institute of Animal Sciences and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China)
出处
《中国动物传染病学报》
CAS
北大核心
2017年第1期80-86,共7页
Chinese Journal of Animal Infectious Diseases
基金
科技部畜禽重大疫病防控与高效安全养殖综合技术研发重点专项(2016YFD0500900)
江苏高校优势学科建设工程资助项目
重大动物疫病防控技术引进(国家农业部948计划
2011-G24)
江苏省属高校自然科学重大基础研究项目(14KJA230001)
江苏省农业支撑项目(BE2014358)
泰州市农业科技计划项目(TZ201421)
关键词
牛病毒性腹泻病毒
分离株
检测
鉴定
全基因组
Bovine viral diarrhea virus
isolates
detection
identifi cation
complete genome
