摘要
目的探讨维生素D受体TaqI基因多态性与儿童肾病综合征激素治疗敏感性的关系.方法研究对象为2015年5月至2016年11月收住昆明医科大学第一附属医院儿科的70例肾病综合症患儿,维生素D受体TaqI基因多态性检测方法为PCR扩增产物酶切后琼脂糖凝胶电泳检测.结果在70例肾病综合征患儿中,其中激素敏感型42例,激素耐药型28例.在42例激素敏感型肾病综合症患儿中,TT基因型频率57.1%,在28例激素耐药型肾病综合症患儿中,TT基因型频率57.1%,(P=1.000).在42例激素敏感型肾病综合症患儿中,Tt基因型频率26.2%,在28例激素耐药型肾病综合症患儿中,Tt基因型频率25.0%,(P=0.911).在42例激素敏感型肾病综合症患儿中,tt基因型频率16.7%,在28例激素耐药型肾病综合症患儿中,tt基因型频率17.9%,(P=0.897).在42例激素敏感型肾病综合症患儿中,T基因频率70.2%,t基因频率29.8%,在28例激素耐药型肾病综合症患儿中,T基因频率69.6%,t基因频率30.4%,(P=0.096).等位基因型频率与等位基因频率在激素敏感型与激素耐药型之间无统计学意义.结论维生素D受体TaqI基因多态性不能作为肾病综合症的标志基因,也不能预测肾病综合症患儿对激素治疗的反应性.不能作为激素耐药型肾病综合征的标志基因.
Objective To determine the frequency of gene and the association of VDR gene polymorphismswith nephrotic syndrome(NS)and steroid responsiveness in Yun Nan children,so as to guide the use of hormone and immune inhibitor in this disease.Methods The subjects of the study were the70cases of children with nephrotic syndrome in pediatrics in the first affiliated hospital of kunming medical university from2015,May to2016,November.Genotypes of the VDR TaqI gene polymorphism were analyzed using the PCR amplification product enzyme and agarose gel electrophoresis.Results A total of70NS(42steroid sensitive[SS]and28steroid resistant[SR])patientswith a mean age of9.14±3.80years were studied.The TT genotypeofVDR-TaqI polymorphism was detected in57.1%of the SS patients compared to57.1%of SR patients(P=1.000).The Tt genotype was detected in26.2%of SS patientscompared to25.0%of SR patients(=0.911).The tt genotype was detected in16.7%of SS patients compared to17.9%of SR patients(P=0.897).The T-allele frequency was70.2%in SS patients compared to69.6%of SR patients.The t-allele frequency was29.8%in SS patients compared to30.4%of SR patients(P=0.096).No significant difference was detected in terms of VDR-Apal polymorphism in SS patients compared to SR patientsfor all the three genotypes(P=1.000,P=0.911,and P=0.897).No significant difference was found in any of the allele frequencies between SS and SR(P=0.096).Conclusion Vitamin D Receptor Gene TaqI polymorphisms has no correlation with the steroid responsiveness in childhood nephrotic syndrome.
作者
崔坤华
李琪
CUI Kun-hua;LI Qi(Dept. of Pediatrics,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)
出处
《昆明医科大学学报》
CAS
2017年第10期60-64,共5页
Journal of Kunming Medical University
基金
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(AFRP2014)
