摘要
【目的】研究新疆东部棉花根茎部病害的种类及分布,为当地高效防控此类病害提供依据。【方法】采用已报道的4种引起棉花根茎部病害的主要病原菌(尖孢镰孢霉、立枯丝核菌、大丽轮枝菌和拟轮枝镰孢霉)的特异性引物,特异性验证后,分别对新疆东部棉花区按不同以村(连)为单位采集的混合病样进行PCR检测。【结果】在检测灵敏度为0.5 ng/μL的检测体系中,从22份新疆东部棉花病株混合样品中可以检测出4种病原菌中的3种,分别为尖孢镰孢霉、立枯丝核菌、大丽轮枝菌;其中尖孢镰刀菌检出率最高,立枯丝核菌检出率次之,大丽轮枝菌检出率较低;而拟轮枝镰孢霉在22份混合病样中的检出率0%。【结论】枯萎病、立枯病和黄萎病是新疆东部根茎部主要病害;其中枯萎病的发生最为普遍,立枯病次之,黄萎病发生的普遍率相对较低,红腐病在新疆东部无发生或发生分布极少。
【Objective】Clarifying species and distribution of cotton root and stem diseases in eastern Xinjiang can provide a basis for effective prevention and control of such diseases in the local area.【Method】The reported specific primers of the4species pathogens(Fusarium oxysporum,Rhizoctonia solani,verticillium dahliae and Fusarium verticilliodes)which cause the root and stem disease of cotton are used for the specific verification,and carrying out PCR detection for the mixed disease samples of the collected according to different villages(townships)from the cotton areas in Eastern Xinjiang.【Result】In the tested system with sensitivity of0.5ng/μL,3species of4pathogenic bacteria were detected from the22mixed samples of cotton infected plants from the Eastern Xinjiang.They were respectively Fusarium oxysporum,Rhizoctonia solani and verticillium dahliae;Among them,Fusarium oxysporum had the highest detection rate,Rhizoctonia solani was the second,V.dahliae dad the lower detection rate.But,the detection rate ofFusarium verticilliodes was0%in the22mixed samples.【Conclusion】The detection results indicated that the Fusarium wilt,Rhizoctonia damping-off and Verticillium yellow wilt were the main root and stem diseases of cotton in Eastern Xinjiang;Among which,Fusarium wilt is the most common one,followed by Rhizoctonia damping-off,and the incidence of Verticillium wilt is relatively low.In Eastern Xinjiang,red rot has not occurred or distribute very little.
作者
马依努尔.米吉提
族米娜
萨吉达.艾孜则
郭庆元
Mayinuer Mijiti;Zumina;Sajida Aizizi;GUO Qing -yuan(Xinjiang Agricultural University / College of Agronomy / Key Laboratory of the Agriculture and Forestry Pest Monitoring and Safety Control of College of Agronomy, Xinjiang Agricultural University,Urumqi 830052, China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2017年第11期2046-2053,共8页
Xinjiang Agricultural Sciences
基金
农业部专项"作物根腐类病害防控技术研究与集成"(201503112)
新疆维吾尔自治区重点研发计划"棉田主要病虫防治及农药减施增效新技术与产品研发"(2016B01001)~~
关键词
棉花
根茎部病害
PCR检测
病害分布
cotton
root and stem disease
PCR detection
distribution of disease
