摘要
目的观察泽泻汤(RAD)对高脂血症大鼠肝脏过氧化物酶体增殖物激活受体α(PPARα)和乙酰辅酶A氧化酶(ACO)基因和蛋白表达的影响,对泽泻汤防治高脂血症的作用机制进行初步探讨。方法采用喂饲高脂饲料方法复制高脂血症模型,然后分别给予泽泻汤高、低剂量及血脂康胶囊治疗,4周后,检测血清TC、TG、HDL和LDL的含量,利用RT-PCR和Western blot的方法观察肝脏PPARα和ACO的基因和蛋白的表达,HE方法观察肝组织形态学改变。结果泽泻汤能明显降低血清TC、TG及LDL的含量(P<0.01),升高HDL含量(P<0.01),增强肝脏PPARα和ACO的表达(P<0.01),改善大鼠脂肪变性程度。结论泽泻汤增强肝组织中PPARα和ACO的表达可能是其防治高脂血症的机制之一。
AimTo primarily discuss the mechanism of Rhizoma Alismatis decoction on prevention and treatment of hyperlipemia,through investigating hepatic expressions of peroxisome proliferator activated receptorα(PPARα)and acyl CoA oxidase(ACO)in rats with hyperlipemia.MethodsFifty male Sprague Dawley rats(160~180g)were randomly divided into five groups:control group,model group,high dose of Rhizoma Alismatis decoction group(H RAD),low dose of Rhizoma Alismatis decoction group(L RAD),and Xue Zhi Kang group(XZK).Rats in control group were fed with ordinary forage,and the others were with high fat forage,which lasted for four weeks.At the same time,high and low dose of Rhizoma Alismatis decoction,as well as Xuezhikang capsule,was administered in respectively designed groups.Then,the TC,TG,HDL and LDL of serum were detected,the morphology of liver tissues was observed with HE,and the expressions of PPARαand ACO were detected with RT PCR and Western blot after four weeks.ResultsRhizoma Alismatis decoction could significantly reduce serum concentration of TC,TG and LDL(P<001),while increasing the concentration of HDL(P<001)and strengthening the expressions of PPARαand ACO(P<001).ConclusionStrengthening the expressions of PPARαand ACO can be viewed as mechanisms of Rhizoma Alismatis decoction in prevention and treatment of hyperlipemia.
作者
韩雪
张睦清
常冰
王茜
郝蕾
刘宇
石铖
郭秋红
张一昕
HAN Xue;ZHANG Mu qing;CHANG Bing;WANG Xi;HAO Lei;LIU Yu;SHI Cheng;GUO Qiu hong;ZHANG Yi xin(Dept of Clinic Chinese Medicine, School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang050200,China;Dept of Cardiovasology, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang050017,China;Dept of Cardiovasology, Shijiazhuang City Hospital of Traditional Chinese Medicine, Shijiazhuang050017, China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2017年第12期1761-1765,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81503268)
河北省高等学校科学技术研究项目(No QN2015251)
河北省中医药管理局科研计划项目(No 2015077)
国家级大学生创新项目(No 201614432002)
