摘要
以巯基乙酸和巯基乙酰肼为稳定剂,制备了酸度敏感型CdTe量子点。经与抗体链接,该量子点具备酸度敏感、免疫识别双重靶向功能。经荧光光谱分析、透射电镜图像及细胞免疫成像证明,抗体已成功链接于量子点表面,且该量子点具有酸度敏感及抗体识别的双重靶向功能,可以实现对肿瘤细胞的特异性标记。
The pH sensitive CdTe quantum dots(Q D s)were prepared with thioglycolic acid(T G A)and mercapto-acetohydrazide(T G H)as the stabilizers.After being conjugated with antibody,the prepared CdTe QDs has both functions of pH sensitivity and immune recognition.The results of fluorescence luminescence(F L),transmission electron microscopy(T E M)and cells labeling imaging showed that antibody was successfully conjugated on the surface of CdTe QDs.The prepared dual targeting QDs can be used to identify the acidic interstitial extracellular environment and label tumor cells specifically.
作者
杨冬芝
王文文
杜岩
纪海侠
YANG Dongzhi;WANG Wenwen;DU Yan;JI Haixia(Department of Pharmaceutical Analysis , Xuzhou Medical University , Xuzhou 221004, Jiangsu,P. R. China;Jiangsu Key Laboratory of Nezv Drug Research and Clinical Pharmacy ,Xuzhou Medical University ,Xuzhou 221004, Jiangsu,P. R. China)
出处
《影像科学与光化学》
CAS
CSCD
北大核心
2017年第6期780-787,共8页
Imaging Science and Photochemistry
基金
国家自然科学基金(81201696)
江苏省自然科学基金(BK20161173)
国家大学生创新创业训练计划(20131031300)
江苏省高等学校大学生实践创新训练计划项目(201310313004Z)
