摘要
分离自青海湖周边土壤的菌株246-1平板对峙实验显示了对黄瓜枯萎病菌(Fusarium oxysporum f.sp.cucumerinum)的抑制作用。通过16S rDNA鉴定其为多黏类芽孢杆菌(Paenibacillus polymyxa)。实验通过筛选fusaricidins提取纯化和检测方法,确定发酵液酸化是fusaricidins提取纯化的一个重要步骤;同时确定乙腈/水/TFA检测体系为fusaricidins HPLC检测流动相。采用抑菌圈实验筛选出最适合多黏类芽孢杆菌246-1产fusaricidins的培养基,用单因素实验方法对其培养条件进行优化。结果表明,246-1菌株的培养基配方为可溶性淀粉17.5 g/L,酵母浸出粉4 g/L,蛋白胨25 g/L,NaCl 1.25 g/L,Na_2HPO_40.5 g/L,CaCl_21g/L,蔗糖15 g/L,最佳培养时间48 h,初始pH 7.0,培养温度30℃。进一步分析了多黏类芽孢杆菌246-1对黄瓜幼苗的促生作用,在黄瓜幼苗根际的定殖能力和对黄瓜枯萎病的防效作用。盆栽试验结果表明,菌株246-1的发酵上清液对黄瓜枯萎病菌表现出很强的抑制作用,同时该菌株在黄瓜幼苗根际具有很好的定殖能力,表现了对黄瓜幼苗的显著促生效果。
Strain246-1,which was isolated from the soil beside of Qinghai Lake,had a high antagonistic activity against F.oxysporum f.sp.cucumerinum.It was identified as Paenibacillns polymyxa based on the16S rDNA sequence.The extraction and detection methods of fusaricidins were screened and,the fermented liquid acid was proved to be an important step of fusaricidins extraction and purification.The acetonitrile/water/TFA detection system was used for detecting fusaricidins by HPLC.We selected the most suitable medium for P.polymyxa246-1to produce fusarcidins by using the inhibition zones experment and designed according to single factor experment.The optimal medium for strain246-1consisted of17.5g·L1soluble starch,4g·L1yeast extract powder,25g·L_1peptone,1.25g·L_1NaCl,0.5g·L_1Na2HPO4,CaCL21g·L_1,15g·L-1sucrose.And thefer mentation time is48h,pH7.0and30℃Further analysis was conducted includingthegrowth of cucumber seedlings influenced by strain246-1,rhizosphere colonization ability of246-1and its control effect on cucumber wilt disease.The results of pot experment showed that,strain246-1had significant biocontrol effects against fusarum wilt disease in cucumber seedlings resulted from the C.oxyssoriim f.sp.cucumerinum.It also canpromote the growth of cucumber seedlings and displayed strong colonization ability in the rhizosphere of cucumber seedlings.
作者
白雪莲
吴慧玲
张虹
刘伟成
敖恩宝力格
Bai Xuelian;Wu Huiling;Zhang Hong;Liu Weicheng;Aoen Baolige(College of life science and technology,Inner Mongolia Normal University, Hohhot 010022,P. R. China;Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097,P. R. China)
出处
《科学技术与工程》
北大核心
2017年第33期69-76,共8页
Science Technology and Engineering
基金
北京市农林科学院青年科研基金(QNJJ201411)
北京市农林科学院科技创新能力建设专项(KJCX20170410)
北京市优秀人才青年骨干个人项目(2014000020060G180)资助
