摘要
[目的]建立测定保心宁片中人参皂苷Rg_1含量的方法。[方法]Hypersil-ODS(150 mm×4.6 mm,5μm)色谱柱,甲醇∶水(45∶55)为流动相,流速1.0 mL/min,检测波长203 nm。[结果]人参皂苷Rg_1在1.39~48.79μg范围内具良好的线性关系,其线性方程为A=231 896a+44 038(n=10,r=0.999 4),平均回收率为100.9%,RSD=0.87%(n=5)。[结论]HPLC法测定人参皂苷Rg_1的含量线性关系好、准确度高、稳定性好、重复性好,可作为该药品的质量控制方法。
〔Objective〕To establish an HPLC method for the determination of ginsenoside Rg1in Baoxinning Tablets.〔Methods〕The sample was analyzed on a Hypersil ODS chromatogram column(150mm×4.6mm,5μm),with mobile phase of methanol water(45∶55)at flow rate of1.0ml/min,and wavelenghth at203nm.〔Results〕A linearity of ginsenoside Rg1was good at1.39to48.79μg.The average recovery was100.9%,with RSD0.87%(n=5).〔Conclusion〕It showed that this method had a good stability,precision and repeatability.It was very significant for the quality control of Baoxinning Tablets.
作者
王先友
郭石
WANG Xianyou;GUO Shi(Pharmaceutical College of Henan University,Kaifeng 475002,China;Children Hospital of Kaifeng,Kaifeng 475002,China)
出处
《河南大学学报（医学版）》
CAS
2017年第4期263-264,共2页
Journal of Henan University:Medical Science
