摘要
目的观察槟榔提取液(areca nut extract,ANE)对口腔黏膜成纤维细胞Ⅰ型胶原分泌的诱导作用及扶正活血解毒方的干预作用。方法体外培养口腔黏膜成纤维细胞,ANE为诱导剂,扶正活血解毒中药药物血清(低、中、高)为干预物;用免疫细胞化学法检测细胞内Ⅰ型胶原的表达,用酶联免疫吸附剂测定法(ELISA)检测培养上清Ⅰ型胶原的含量。结果 ANE对口腔黏膜成纤维细胞Ⅰ型胶原的合成有促进作用(P<0.01),扶正活血解毒方含药血清(中、高)对ANE刺激下的FB胶原合成有抑制作用(P<0.05)。结论扶正活血解毒中药可下调ANE诱导的口腔黏膜成纤维细胞Ⅰ型胶原的表达。
Objective To observe the expression of collagen I in oral mucosal fibroblasts induced by ANE and the intervention of Fuzheng Huoxue Jiedu decoction.Methods Oral mucosal fibroblasts were cultured in vitro,ANE was used as an indicator,and Fuzheng Huoxue Jiedu decoction as intervention medicine.The expression of collagen I was measured by immunocytechemical method,and the content of collagen I was determined by ELISA.Results ANE had promotion effect on the synthesis of collagen I in oral mucosal fibroblasts(P<0.01),Fuzheng Huoxue Jiedu decoction drug serum had inhibition effect on the expression of collagen I induced by ANE(P<0.05).Conclusion Fuzheng Huoxue Jiedu decoction can inhibit the expression of collagen I in oral mucosal fibroblasts induced by ANE.
作者
罗玉姣
岳金宝
吴丹
刘寻
李群
谭劲
LUO Yujiao;YUE Jinbao;WU Da;LIU Xun;LI Qun;TAN Jin(The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China)
出处
《湖南中医药大学学报》
CAS
2017年第12期1308-1311,共4页
Journal of Hunan University of Chinese Medicine
基金
国家自然基金项目(81373701)
