摘要
目的研究血浆葡萄糖在不同糖酵解抑制剂及抗凝剂管中室温放置24h条件下的稳定性。方法对20例志愿者每人采集6支不同糖酵解抑制剂-抗凝剂管血样,采样后室温放置0、2、4、8、24h后分别测定各管血浆葡萄糖,比较每种样本管放置不同时间葡萄糖水平差异及不同时相样本管血浆葡萄糖水平的差异。结果 6样本管2h血糖结果与即时测定(0h)结果比较,差异有统计学意义(P<0.05);含氟化钠的3样本管放置相同时间血糖比较,差异无统计学意义(P>0.05),3样本管采样后2、4、8、24h血糖结果多重比较,差异无统计学意义(P>0.05);含碘乙酸钠3样本管放置不同时间血糖比较,差异均有统计学意义(P<0.05),放置相同时间3样本管血糖比较,采样后0、2、4小时差异均无统计学意义(P>0.05),采样后8、24h差异有统计学意义(P<0.01);其他条件相同,两种糖酵解抑制剂管血糖水平比较,除采样后放置2h差异无统计学意义外(P>0.05),其余时间点的差异均有统计学意义(P<0.01)。结论室温放置24h内氟化钠管更适于血浆血糖检测,抗凝剂种类与抑制糖酵解能力无明显相关。
Objective To study the stability of the plasma glucose in sample tubes containing different glycolysis inhibitors and anticoagulants at room temperature for up to24h.Methods Venous blood was collected from20volunteers.Each donor blood sample was divided into six tubes containing different glycolysis inhibitors and anticoagulants and kept at room temperature.Plasma glucose was measured at0,2,4,8and24h after collection.The difference of plasma glucose levels for each sample tube was compared with different placed time and the difference of plasma glucose levels for the same placed time was compared with different sample tubes.Results The differences of the plasma glucose results of2hours after sampling and of instant detecting are statistically significant for each sample tube(P<0.05).The differences of the plasma glucose levels among the three sample tubes containing sodium fluoride are not statistically significant at the same placed time(P>0.05).The differences of the plasma glucose results of2,4,8,24h after sampling for each sample tube are not statistically significant(P>0.05).The differences of the plasma glucose results for each sample tube containing sodium iodoacetate are statistically significant at different placed times(P<0.05).The differences of plasma glucose levels of0,2,4hours after sampling for the three sample tubes are no statistically significant(P>0.05)and the differences of plasma glucose levels of8,24hours after sampling for the three sample tubes are statistically significant(P<0.05).The differences of plasma glucose levels for the two glycolytic inhibitors are statistically significant at4,8,24hours after sampling(P<0.01)except2hours after sampling(P>0.05).Conclusion Tubes containing NaF are more suitable for detecting venous plasma glucose within24h at room temperature.The type of anticoagulant has no obvious correlation with the ability of glycolytic inhibition.
作者
罗国霞
李广权
LUO Guoxia;LI Guangquan(Department of Laboratory Medicine,Fuling Central Hospital of Chongqing,Fuling,Chongqing 408000,China;Department of Laboratory Medicine,No.363 Hospital,Chengdu,Sichuan 610041,China)
出处
《国际检验医学杂志》
CAS
2017年第24期3410-3412,共3页
International Journal of Laboratory Medicine
