摘要
目的:探究不同剂量的乌贼墨汁黑色素对链脲佐菌素(streptozocin,STZ)诱导的糖尿病肾病模型小鼠肾脏的保护作用及其机制。方法:采用腹腔注射STZ方法建立糖尿病肾病小鼠模型,将实验小鼠分为正常组、模型组、阳性治疗组(盐酸二甲双胍120 mg/kg,以体质量计,下同)、黑色素低(120 mg/kg)、中(240 mg/kg)、高(480 mg/kg)剂量组,连续灌胃4周,测定肾脏指数、肾功能、肾脏抗氧化体系相关酶活力;组织切片观察肾脏形态变化;实时荧光定量聚合酶链式反应(real time polymerase chain reaction,RT-PCR)测定诱发肾脏纤维化细胞因子的mRNA表达量。结果:与模型组相比,乌贼墨汁黑色素中、高剂量组能极显著增强肾功能(P<0.01),提高抗氧化酶活性(P<0.05或P<0.01),减少丙二醛含量(P<0.05或P<0.01),降低肾纤维化转化生长因子及其受体mRNA的表达(P<0.01),上调抗炎性因子过氧化酶体增殖物活化受体mRNA表达量(P<0.01);高剂量组肾小管基底及系膜区细胞外基质积聚减轻,肾小管间质纤维化程度下降;低剂量组无显著差异。结论:中、高剂量的乌贼墨汁黑色素能减轻糖尿病肾病的肾发炎,增强肾功能,其作用机制可能与提高肾脏抗氧化能力,抑制肾纤维化细胞因子的过度表达相关。
Objective:To determine the protective effect and underlying mechanism of melanin extracted from cuttlefish ink sacs on streptozocin-induced diabetic nephropathy in mice.Methods:Diabetic nephropathy was induced in mice by intraperitoneal injection of streptozocin(STZ).The experimental mice were randomly divided into6groups:normal control,model control,positive control,low-dose(120mg/kg),medium-dose group(240mg/kg),and high-dose melanin(480mg/kg)groups.After four weeks of oral administration,kidney index,kidney function and antioxidant enzymes activities were measured,and the kidneys were histologically examined.The mRNA expression of renal fibrogenic cytokines was detected by real time polymerase chain reaction(RT-PCR).Results:Compared with the model control group,the medium-and highdosagegroups showed significantly enhanced kidney function(P<0.01),increased antioxidant enzyme activities(P<0.05or P<0.01),reduced mRNA expression of transforming growth factor-β1(TGF-β1)and its receptors,and increased mRNA expression of peroxisome proliferators-activated receptor-g(PPAR-g)(P<0.01).Moreover,high-dosage melanin reduced extracellular matrix accumulation in the basal and mesangial regions of renal tubular cells and decreased tubulointerstitial fibrosis,while no significant differences were observed for the low-medium melanin group.Conclusion:Melanin from cuttlefish ink can relieve diabetic renal inflammation,likely by enhancing renal antioxidant capacity and inhibiting the overexpression of renal fibrogenic cytokines.
作者
董慧
徐洋洋
王春琳
宋微微
母昌考
李荣华
DONG Hui;XU Yangyang;WANG Chunlin;SONG Weiwei;MU Changkao;LI Ronghua(Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第5期192-198,共7页
Food Science
基金
浙江省自然科学基金项目(LY18C190006)
浙江省公益性项目(ZX2013000403)
宁波大学"水产"重中之重学科开放基金项目
宁波市重大农业专项(201401C1111001)
大学生创新孵化项目(2016R405096)
关键词
乌贼墨汁黑色素
糖尿病肾病
细胞因子
melanin extracted from cuttlefish ink sacs
diabetic nephropathy
cytokines
