摘要
以亚硒酸钠为硒化剂,在Ba Cl_2的催化下对肿节风浸膏残渣粗多糖进行硒化修饰,以产率和硒含量作为考察指标,采用正交设计方法对硒化肿节风浸膏残渣多糖的制备工艺进行优化。同时对制备的硒化肿节风浸膏残渣多糖进行了纯度、相对分子质量、红外光谱、热重学性质和体外抗肿瘤活性研究。结果表明,硒化修饰的最佳工艺条件为:反应温度70℃、反应时间7 h、HNO_3质量分数1.0%、Ba Cl_2加入量1.5 g,在此条件下制备所得的硒化肿节风浸膏残渣多糖总糖含量为(94.89±0.83)%,产率为(21.93±0.85)%,硒元素含量为(944.54±13.91)μg/g,相对分子质量为1 339 k D。体外抗肿瘤实验结果表明,硒化肿节风浸膏残渣多糖比原肿节风浸膏残渣粗多糖具有更高的抗肿瘤活性,有进一步开发为新型抗肿瘤药物的潜力,从而提高肿节风浸膏残渣的利用率。
Selenylation modification of polysaccharides from the extract residue of Sarcandra glabra(Thunb.)Nakai was operated with sodium selenite as modification agent and barium chloride as the catalyst.The synthesis conditions were optimized by orthogonal experiment with the yields and contents of selenium as indicators.Then,the purity,molecular weight,infrared spectroscopy,thermophysical properties and antitumor activity of the selenylated polysaccharides were determined.Results showed that the optimum conditions for preparing selenylated polysaccharides from the extract residue of S.glabra(Thunb.)Nakai were at70°C for7h with1.0%nitric acid using1.5g barium chloride as catalyst.Under the optimum condition,the physicochemical properties of the derivatives were measured and the results showed that the total carbohydrate content was(94.89±0.83)%,the yield was(21.93±0.85)%,the content of selenium was(944.54±13.91)μg/g,the molecular weight was1339kD.In vitro antitumor activity indicated that exposure of HepG2cells to the increasing concentrations of the crude polysaccharide from the residue of S.glabra(Thunb.)Nakai and its selenylated products decreased cell viability in dose-dependent and time-dependent manner.The selenium polysaccharide demonstrated a better antitumor activity compared with the raw polysaccharide,which could be explored as a potential antitumor drug from abandoned extracted residue for the functional foods and pharmaceutical industries.
作者
胡成
刘雅萌
戴士杰
刘玮
高向东
HU Cheng;LIU Yameng;DAI Shijie;LIU Wei;GAO Xiangdong(Jiangsu Key Laboratory of Druggability of Biopharmaceuticals,School of Life Science and Technology,China Pharmaceutical University, Nanjing 210009,China)
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2018年第1期109-116,共8页
Journal of China Pharmaceutical University
基金
国家自然科学基金资助项目(No.31301473
No.81473216
No.81673589)
国家重大新药创制重大专项资助项目(No.2012ZX09502001-004)
江苏省高校青蓝工程资助项目
江苏省高校优秀科技创新团队资助项目~~
关键词
肿节风
浸膏残渣
硒化多糖
制备
抗肿瘤活性
S.glabra (Thunb.) Nakai
extract residue
selenylated polysaccharides
preparation
antitumor
