摘要
目的:研究超微粉碎技术对灵芝中三萜类成分溶出的影响。方法:采用紫外-可见分光光度法测定灵芝中三萜类成分总提取率,高效液相色谱法(HPLC)测定灵芝中9种三萜类成分(灵芝酸A、灵芝酸B、灵芝酸C、灵芝酸C1、灵芝酸C2、灵芝酸D、灵芝酸E、灵芝酸G、灵芝酸H)含量,比较30、50、80目灵芝普通粉和300目灵芝超微粉中三萜类成分总提取率及9种三萜类成分的含量。结果:30、50、80、300目灵芝粉中三萜类成分总提取率分别为(0.74±0.08)%、(0.75±0.06)%、(0.78±0.06)%、(1.09±0.10)%(RSD<2%,n=3);随着灵芝粉目数的增大,三萜类成分含量逐渐增大,其中300目含量最大,显著高于灵芝普通粉(P<0.05)。结论:灵芝经超微粉碎后三萜类成分的溶出增加。
OBJECTIVE:To study the effects of ultrafine grinding technology on the dissolution of triterpenoids from Ganoderma lucidum.METHODS:UV spectrophotometry was used to determine total extration rates of triterpenoids from G.lucidum.HPLC method was used to determine the contents of 9 kinds of triterpenoids(ganoderic acid A,ganoderic acid B,ganoderic acid C,ganoderic acid C1,ganoderic acid C2,ganoderic acid D,ganoderic acid E,ganoderic acid G,ganoderic acid H)from G.lucidum.The total extration rates and the contents of 9 kinds of triterpenoids were compared between 30,50,80 mesh common G.lucidum powder and 300 mesh G.lucidum ultramicro powder.RESULTS:The total extraction rates of triterpenoids from 30,50,80,300 mesh G.lucidum powder were(0.74±0.08)%,(0.75±0.06)%,(0.78±0.06)%,(1.09±0.10)%(RSD<2%,n=3),respectively.With the increase of the mesh number of G.lucidum powder,the contents of triterpenoids were increased gradually,and were the highest in 300 mesh,which was significant higher than common G.lucidum powder(P<0.05).CONCLUSIONS:After ultrafine grinding,the dissolution of triterpenoids from G.lucidum is increased.
作者
张福君
瞿晶田
王强
ZHANG Fujun;QU Jingtian;WANG Qiang(Dept.of Pharmacy,the First Affiliated Hospital to Tianjin University of TCM,Tianjin 300192,China)
出处
《中国药房》
CAS
北大核心
2018年第5期599-602,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81603648)
