摘要
目的优选藏药柳茶总三萜的提取富集工艺并研究其抗缺氧活性。方法通过小鼠常压密闭缺氧实验筛选柳茶总三萜的提取溶剂;再以总三萜含量、提取率为评价指标,采用正交试验考察提取时间、提取次数、料液比,优选柳茶总三萜的提取工艺;然后考察大孔吸附树脂类型、上样浓度、洗脱剂浓度、洗脱剂体积,优选其富集工艺;最后通过小鼠常压密闭缺氧实验和急性减压缺氧实验研究其抗缺氧活性。结果优选的提取工艺为:70%乙醇提取,料液比为1∶20,提取2次,每次1.5 h,平均总三萜含量可达32.25%;优选的富集工艺为:AB-8型大孔吸附树脂,上样浓度为2 mg/m L,80%乙醇5 BV洗脱,总三萜含量可达61.09%;柳茶总三萜抗缺氧活性较强,且存在一定的剂量依赖性。结论本研究优选的柳茶总三萜提取富集工艺稳定、重现性好,所得柳茶总三萜具有显著的抗缺氧活性。
Objective To optimize the extraction and enrichment process of total triterpenes from Sibiraea Angustata;To study its anti-hypoxia activity.Methods The extraction solvent of total triterpenoids of Sibiraea Angustata was screened by atmospheric pressure anti-hypoxia test.The content of total triterpenes and extraction rate were set as evaluation indexes.The effects of extraction time,extraction times and ratio of material to liquid on extraction were investigated by orthogonal test.The extraction process was optimized.Resin type,concentration of sample,eluent concentration and eluent volume were investigated to optimize the enrichment process of total triterpenes of Sibiraea Angustata.Finally,the anti-hypoxia activity was studied by atmospheric pressure test and acute decompression test.Results The optimum extraction process was as follows:70%ethanol extraction,the ratio of material to liquid was1:20,extrat2times with1.5h for each time,and the content of total triterpenes was32.25%.The optimum enrichment process was:AB-8-type macroporous adsorption resin,the loading concentration of2mg/mL,80%ethanol5BV elution,and total triterpenes content was up to61.09%;Sibiraea Angustata total triterpenes anti-hypoxia activity test was strong,and had a certain dose dependency.Conclusion The extraction and enrichment process of total triterpenes from Sibiraea Angustata is stable,reproducible and has significant anti-hypoxia activity.
作者
王昕
葸慧荣
杨芳芳
何蕾
马慧萍
WANG Xin;XI Hui-rong;YANG Fang-fang;HE Lei;MA Hui-ping(Pharmacy College of Gansu University of Chinese Medicine, Lanzhou 730000, China;Key Laboratory of Prevention and Cure for the Plateau Environment Damage of PLA, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, China)
出处
《中国中医药信息杂志》
CAS
CSCD
2018年第4期71-76,共6页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81571847
81202458)
甘肃省自然科学基金(145RJZA089)
全军医药卫生科研基金(CLZ14JA01)
关键词
柳茶
总三萜
提取工艺
富集工艺
抗缺氧
Sibiraea Angustata
total triterpenes
extraction process
enrichment process
anti-hypoxia
