摘要
目的:观察链霉蛋白酶能否减少幽门螺杆菌在胃内定植的数量。方法:取诊断为胃恶性肿瘤且呼气试验阳性患者的术后标本,置于生理盐水、链霉蛋白酶及乙酰半胱氨酸中浸泡30 min,经免疫组化染色后制成切片,计算片切幽门螺杆菌数量及粘液厚度。结果:乙酰半胱氨酸组与链霉蛋白酶组比较、链霉蛋白酶组与生理盐水组比较差异均具有统计学意义(P<0.05)。乙酰半胱氨酸组、链霉蛋白酶组粘液层厚度均明显小于生理盐水组(P<0.05),乙酰半胱氨酸组粘液层厚度明显小于链霉蛋白酶组(P<0.05)。结论:链霉蛋白酶组对于减少粘液层厚度优于乙酰半胱氨酸及生理盐水,链霉蛋白酶在减少粘液厚度及减少细菌数量的效果优于生理盐水,不弱于乙酰半胱氨酸,为今后进一步应用研究链霉蛋白酶辅助根除幽门螺杆菌提供了理论依据。
Objective:To observe whether pronase could reduce the number of planted helicobacter pylori in stomach.Methods:The postoperative samples of patients diagnosed with gastric malignancy and positive breath test were collected,put into normal saline,pronase and acetylcysteine to be immersed for 30 min,and then sliced after immunohistochemical staining,so as to calculate the number of helicobacter pylori and mucous thickness in the slide.Results:There were significant differences between acetylcysteine group and pronase group and between pronase group and normal saline group(P<0.05).Both acetylcysteine group and pronase group were notably less than normal saline group in the mucous thickness(P<0.05),and acetylcysteine group was prominently less than pronase group in the mucous thickness(P<0.05).Conclusions:Pronase is better than acetylcysteine and normal saline in reducing mucous thickness,and is better than normal saline and not poor to acetylcysteine in reducing mucous thickness and the number of bacteria,thus providing a theoretical basis for further application of streptomycin to assist in the eradication of Helicobacter pylori.
作者
董浩
金世禄
朱凤鸣
路敏敏
DONG Hao;JIN Shi-lu;ZHU Feng-ming;LU Min-min(Department of Gastroenterology,Binzhou People’s Hospital Affiliated to Binzhou Medical University,Binzhou 256610,Shandong,China)
出处
《川北医学院学报》
CAS
2018年第1期118-120,共3页
Journal of North Sichuan Medical College
关键词
霉蛋白酶
幽门螺杆菌
胃癌
Pronase
Helicobacter pylori
Gastric cancer
