摘要
该研究采用方差分析、相关性分析和聚类分析等方法,对105份杜仲种质资源叶片6种主要活性成分含量进行比较分析,探讨其遗传变异特征,为叶用杜仲的良种选育及开发利用提供理论依据。结果显示:(1)不同种质间杜仲叶片6种活性成分含量的变异程度不同,异槲皮苷含量变异系数(34.42%)最大,变异幅度为1.16~6.92mg·g^(-1),总黄酮变异系数(19.35%)最低,变异幅度为6.70~22.53mg·g^(-1);6种活性成分多样性指数较高,均在2.0以上;不同种质间杜仲叶6种活性成分含量差异性均达到极显著水平(P<0.01)。(2)不同地理来源间杜仲叶片的绿原酸、京尼平苷酸、车叶草苷、异槲皮苷和总黄酮含量存在显著差异。(3)相关性分析表明,3种环烯醚萜类化合物桃叶珊瑚苷、京尼平苷酸及车叶草苷的含量间呈极显著正相关关系;绿原酸与桃叶珊瑚苷、车叶草苷、异槲皮苷、总黄酮间呈极显著正相关关系;总黄酮与异槲皮苷呈极显著正相关关系。(4)根据杜仲叶片6种活性成分的含量聚类,将杜仲种质资源分为4大类群,其中类群Ⅳ为高含量类群,可为优良叶用杜仲资源筛选提供参考。研究表明,杜仲种质资源叶片主要活性成分存在较大变异,表现出丰富的多样性。
Using ANOVA,correlation analysis and cluster analysis,we investigated six active components in leaves of105Eucommia ulmoides germplasm resources,discussed the genetic variation to provide theoretical basis for breeding and utilization of leaf-used E.ulmoides.The results showed that:(1)the variation of six active component contents in leaves were different among different E.ulmoides germplasm resources.The largest coefficient of variation appeared in isoquercitrin(34.42%)and the content ranged from1.16to6.92mg·g-1,while the lowest coefficient of variation appeared in total flavonoids(19.35%)and the content ranged from6.70to22.53mg·g-1.The diversity index of six active components were all above2.0.The contents of six active components showed extremely significant difference(P<0.01)among different germplasm resources.(2)There were significant differences in the contents of chlorogenic acid,geniposidic acid,asperuloside,isoquercitrin and total flavonoids among different origins.(3)Correlation analysis showed that the contents of aucubin,geniposidic acid and asperuloside were extremely significantly related to each other.The content of chlorogenic acid was extremely significant and positive correlated with aucubin,asperuloside,isoquercitrin and total flavonoids.No significant correlation was found between total flavonoids and other components except for isoquercitrin and chlorogenic acid.(4)E.ulmoides germplasm resources were divided into four groups according to the content difference of the six components,in which the groupⅣincluding13materials had higher contents of active components than that of other groups,which could provide basic material for breeding of E.ulmoides resources.Great variation and rich diversity of six active component contents were found in different E.ulmoides germplasm resources.
作者
庆军
魏艳秀
王鼎涵
王璐
刘攀峰
杜红岩
杜庆鑫
QING Jun;WEI Yanxiu;WANG Dinghan;WANG Lu;LIU Panfeng;DU Hongyan;DU Qingxin(Non-timber Forest Research and Development Center of Chinese Academy of Forestry, Zhengzhou 450003, China;The Eucommia Engineering Research Center of National Forestry Administration, Zhengzhou 450003, China;Survey Planning and Design Institute of the State Forestry Administration, Beijing 100010, China;Design Institute of the State Forestry Administration, Beijing 100010, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2018年第2期316-323,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
中央级公益性科研院所基本科研业务费(CAFYBB2016QA017)
"十三五"国家重点研发计划(2017YFD0600702)
关键词
杜仲
种质资源
叶片
活性成分
遗传变异
Eucommia ulmoides
germplasm
leaves
active component
genetic variation
