摘要
构建了以茜素为探针,二氧化钛掺杂乙炔黑和壳聚糖复合材料为信号放大平台的新型免标型沙丁胺醇免疫传感器。于修饰电极的表面电沉积金纳米粒子,实现沙丁胺醇抗体的固定并进一步增大电流响应。采用扫描电子显微镜(SEM)对材料进行表征,循环伏安法(CV)和电化学阻抗谱图(EIS)对修饰电极的构建过程及传感器的性能进行电化学表征。采用差分脉冲伏安法(DPV)检测茜素在修饰电极表面的峰电流值,不同浓度的沙丁胺醇(SAL)抗原与抗体发生免疫亲和反应后,该峰电流值随着沙丁胺醇浓度的增大而减小,并呈线性关系,据此建立峰电流值与沙丁胺醇浓度的关系并实现了对沙丁胺醇的定量检测。探究了缓冲溶液p H值、孵化温度和孵化时间对免疫传感器的影响。在最佳条件下,该免疫传感器对沙丁胺醇的线性响应范围为1.0~100μg/L,检出限(LOD)为0.67μg/L(3σ/k)。该免疫传感器具有良好的重现性、选择性和稳定性,已成功用于猪饲料和猪肉样品中沙丁胺醇的检测,加标回收率分别为100.2%~102.4%(相对标准偏差为1.9%~4.7%)和97.5%~103.3%(相对标准偏差为2.1%~3.5%)。
A novel and label-free electrochemical immunosensor for salbutamol(SAL)detection was developed with alizarin(AR)as a redox probe,and titanium dioxide hybridized acetylene black and chitosan composites(TiO 2-AB-CS)as a signal amplification platform.Au NPs were electro-deposited on the modified electrode surface to immobilize the SAL antibody(anti-SAL),which could further enhance the current response.Scanning electron microscope(SEM)was used to characterize the materials.Cyclic voltammetry(CV)and electrochemical impedance spectroscopy(EIS)were used to characterize the construction process of modified electrode and the electrochemical properties of the immunosensor.Differential pulse voltammetry(DPV)was used to detect the peak current of alizarin on the surface of modified electrode,the peak current of the proposed immunosensor decreased linearly with the increase of SAL concentration when the immune-reaction was carried out with anti-SAL,and the relationship between the peak current and SAL concentration was established to accomplish the quantitative detection on SAL.The experimental factors affecting current response were explored,including pH of phosphate buffer solution(PBS),incubation temperature and incubation time.Under the optimized conditions,the linear range of the immunosensor was obtained to be 1.0-100μg/L with a limit of detection(LOD)of 0.67μg/L(3σ/k).With the advantages of good reproducibility,specificity and stability,the immunosensor was successfully applied in the detection of SAL in swine feed and pork samples with satisfactory results.The recoveries of SAL in swine feed and pork were in the ranges of 100.2%-102.4%and 97.5%-103.3%with relative standard deviations(RSDs)of 1.9%-4.7%and 2.1%-3.5%,respectively.
作者
黄颖
张翠云
HUANG Ying;ZHANG Cui-yun(College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350007,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2018年第2期247-251,共5页
Journal of Instrumental Analysis
基金
福建省科技厅重点项目(JK2010008)
