摘要
为了得到耐高矿化的生物表面活性剂产生菌,采用富集培养,排油圈复筛,从高矿化油田的油水混合物中得到了一株产表面活性剂的菌株K1。通过对K1菌株形态、生理生化特征及16S rRNA基因序列分析,确定该菌为肺炎克雷伯氏菌(Klebsiella pneumoniae)。薄层色谱和红外光谱分析,初步确定该菌株产生的表面活性剂为糖脂类物质。对菌株K1所产表面活性剂在高矿化水中的乳化活性以及温度、酸碱度(pH)对表面活性剂稳定性的影响进行研究,结果显示:在高矿化条件下该生物表面活性剂可以保持较好的乳化活性,对柴油的乳化率为59.5%;具备良好的温度稳定性并可耐受90℃的高温;pH值在6.011.0之间时其活性较强。菌株K1产生的糖脂类生物表面活性剂在提高高矿化油田的原油采收率和原油污染生物修复方面具有应用的潜力。
To obtain efficient halotolerant biosurfactant-producing bacteria,a halotolerant biosurfactant-producing bacteria K1 was isolated from oil-water mixture in high salinity oil field by enrichment cultivation and oil-spreading diameter of culture supernatant.According to morphological,physiochemical characteristics and 16SrDNA sequence,strain K1 was identified as Klebsiella pneumonia.The biosurfactant produced by K1 was identified as Glycolipid by thin layer chromatography and infrared spectroscopy.The emulsifying activity,temperature and pH adaptability of the biosurfactant in highly mineralized water was studied.Experimental results are as follows:The biosurfactant can maintain a good emulsifying activity,and the emulsifying rate of diesel oil by the biosurfactant was 59.5%;it has a good temperature stability and can tolerate the high temperature of 90℃;its activity is stronger when the pH between 6.0~11.0,and peracid greatly reduces its activity.The results show that the biosurfactant producted by strian K1 improves oil recovery in high mineralization oil field and it has a promising potential in bioremediation of crude oil contaminated environments.
作者
李伟
李梦雪
张方荣
王亮
黄建新
LI Wei;LI Mengxue;ZHANG Fangrong;WANG Liang;HUANG Jianxin(College of life Science,Northwest University,Xi′an 710069,China)
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第1期85-90,共6页
Journal of Northwest University（Natural Science Edition）
基金
陕西省自然科学基金资助项目(SJ08ZT08-2)
