摘要
目的通过研究精子顶体精氨酸酰胺酶活性与精子常规和形态之间的关系,评估其在男性不育诊疗中的价值。方法用化学比色法检测精子顶体精氨酸酰胺酶活性,采用计算机辅助分析检测精液常规参数、精子形态精子。结果顶体酶异常组与顶体酶正常组的精子浓度、活动率、前向运动率比较,差异均有统计学意义(P<0.05)。顶体酶正常组与顶体酶异常组精子头部畸形百分率、颈和中段畸形百分率、主段和尾部畸形百分率比较,差异均有统计学意义(P<0.05)。结论精子顶体精氨酸酰胺酶对判断精子质量具有非常高的价值,可作为评价精子功能,辅助诊断男性不育的有效指标。
Objective To evaluate the value of sperm acrosomal arginine enzyme activity in the diagnosis and treatment of male infertility by studying its relationship with sperm motility and morphology.Methods The sperm acrosomal arginine enzyme activity was detected by chemical colorimetric method,and the routine parameters of sperm and sperm morphology were detected by computer-aided analysis.Results There were significant differences on sperm concentration,motility and progressive motility between normal sperm acrosomal arginine enzyme activity group and abnormal sperm acrosomal arginine enzyme activity group(P<0.05).The percentage of deformities in head,neck and middle segment were significant different between normal sperm acrosomal arginine enzyme activity group and abnormal sperm acrosomal arginine enzyme activity group(P<0.05).Conclusion The activity of sperm acrosin is a very efficient marker in sperm quality,and an effective indicator of the evaluation of sperm fertilization potential and the diagnosis of male infertility.
作者
沈丽燕
王家雄
宋丹
程洪波
杨慎敏
王改改
史轶超
SHEN Liyan;WANG Jiaxiong;SONG Dan;CHENG Hongbo;YANG Shenmin;WANG Gaigai;SHI Yichao(Center for Reproduction and Genetics,Suzhou Municipal Hospital Affiliated to Nanjing Medical University,Suzhou,Jiangsu 215002,China)
出处
《国际检验医学杂志》
CAS
2018年第4期426-428,共3页
International Journal of Laboratory Medicine
基金
江苏省遗传医学重点学科基金项目(FXK201305)
苏州市临床重点病种诊疗技术专项项目(lczx201411)
苏州市临床医学中心基金项目(szzx201505)
关键词
精子顶体精氨酸酰胺酶活性
精液参数
精子形态
男性不育
sperm acrosomal arginine enzyme activity
semen parameters
sperm morphology
male infertility
