摘要
目的:研究BCL6共抑制因子(BCOR)异构体(BCOR-short)对根尖牙乳头干细胞(SCAPs)成骨分化能力的影响。方法:利用HA-BCOR-short逆转录病毒载体在SCAPs中过表达BCOR-short,Western Blot检测过表达效果;通过碱性磷酸酶(ALP)活性、茜素红染色及钙离子定量分析研究SCAPs体外成骨分化能力;实时定量RT-PCR检测SCAPs中骨涎蛋白(BSP)、骨钙素(OCN)和转录因子AP2A的表达。结果:Western Blot结果显示HA-BCOR-short可以在SCAPs有效的过表达;ALP活性结果显示过表达HA-BCOR-short抑制SCAPs的ALP活性;茜素红染色及钙离子定量分析结果显示HA-BCOR-short明显抑制SCAPs体外矿化能力;实时定量RT-PCR结果显示过表达HA-BCOR-short明显抑制SCAPs中BSP、OCN和AP2A的表达。结论:过表达BCOR异构体BCOR-short可以抑制转录因子AP2A的表达,并且抑制SCAPs体外成骨分化功能。
Objective:To investigate the effect of BCOR-short on the osteogenic differentiation potential of stem cells from apical papilla(SCAPs).Methods:Retroviral HA-BCOR-short was used to over-express the BCOR-short in SCAPs.Western Blot was used to detect the expression of HA-BCOR-short.Alkaline phosphatase(ALP)activity assay was used to detect the early marker of osteogenic differentiation-ALP activity,Alizarin-red staining and quantitative analysis of calcium were used to investigate the mineralization potential of SCAPs in vitro.Real time RT-PCR was used to detect the osteogenesis related genes expressions bone sialoprotein(BSP),osteocalcin(OCN)and AP2A.Results:Western Blot result showed that HA-BCOR-short could over-express in SCAPs.The over-expression of HA-BCOR-short inhibited ALP activity,mineralization,and the expressions of BSP and OCN in SCAPs.The AP2A expression was repressed in HA-BCOR-short over-expressed SCAPs compared with control group.Conclusions:Our results indicated that over-expression of HA-BCOR-short repressed osteogenic differentiation potential of SCAPs and the expression of AP2A.
作者
王海锋
幸丹
刘亚男
刘思思
宁美芝
李文峰
曹钰
WANG Haifeng;XIN Dan;LIU Yanan;LIU Sisi;NING Meizhi;LI Wenfeng;CAO Yu(Department of Stomatology,Capital Medical University School of Rehabilitation Medicine,China Rehabilitation Research Center,Beijing 100068,China;Department of Integrated Emergency dental care,Capital Medical University School of Stomatology,Beijing 100050,China)
出处
《口腔生物医学》
2018年第1期12-16,共5页
Oral Biomedicine
基金
中国康复研究中心重点课题(2017ZX-03)
