摘要
目的:探讨薏苡附子败酱散对糖尿病小鼠的治疗效果及对炎症因子和肠道菌群的影响。方法:采用链脲佐菌素注射构建糖尿病小鼠模型,随机分为三组:对照组采用生理盐水灌胃治疗,中药组采用薏苡附子败酱散灌胃治疗,他汀组采用辛伐他汀灌胃治疗。治疗28d后收集小鼠血液采用自动生化分析仪分析FPG、TG、TC、HDL、LDL和LPS水平,采用ELISA分析IL-6水平,采用qPCR分析双歧杆菌和乳酸杆菌的数量。结果:和治疗前相比,中药组和他汀组治疗后糖尿病小鼠血清中FPG、TG、TC、LDL、LPS和IL-6的水平显著下降(P<0.05),乳酸杆菌和双歧杆菌的数量显著增加(P<0.05),HDL水平没有显著差异(P>0.05);和对照组治疗后相比,中药组和他汀组治疗后糖尿病小鼠血清中FPG、TG、TC、LDL、LPS和IL-6的水平显著下降(P<0.05),乳酸杆菌和双歧杆菌的数量显著增加(P<0.05),HDL水平没有显著差异(P>0.05);和他汀组治疗后相比,中药组治疗后TG、TC、LDL、LPS、IL-6、乳酸杆菌和双歧杆菌都存在显著差异(P<0.05),而FPG和HDL水平没有显著差异(P>0.05)。结论:薏苡附子败酱散能够增加乳酸杆菌和双歧杆菌的数量,降低LPS和IL-6的水平,改善糖尿病小鼠的糖脂代谢,发挥治疗糖尿病的作用。
Objective:To observe the effect of Yiyi Fuzi Baijiang powder(YFFZBJ)on the diabetes mellitus mice and the changes of inflammatory factor and intestinal flora.Methods:Diabetes mellitus(DM)mice models were induced by injecting streptozotocin.The DM mice divided into three group:Control groupwith physiological saline gavage treatment;Traditional chinese medicine(TCM)group with YFFZBJ gavage treatment;and Statin group with simvastatin gavage treatment;After treatment 28 days,the level of blood sugar,blood fat,and LPS were analyzed by automatic biochemical analyzer,the level of IL-6 were detected by ELISA,and the number of lactobacillus and Bifidobacterium were counted by qPCR.Results:Compared to before treatment,the level of FPG,TG,TC,LDL,LPS,and IL-6 were significantly reduced in TCM group and Statin groupafter treatment(P<0.05),the number of lactobacillus and Bifidobacterium were obviously improved(P<0.05),and the level of HDL had no significantly changed(P>0.05).Compared to control group after treatment,the level of FPG,TG,TC,LDL,LPS,and IL-6 in TCM group and Statin groupafter treatment were significantly reduced(P<0.05),the number of lactobacillus and Bifidobacterium were obviously improved(P<0.05),and the level of HDL had no significantly changed(P>0.05).Compared to Statin group after treatment,the level of FPG,TG,TC,LDL,LPS,IL-6 and the number of lactobacillus and Bifidobacterium in TCM group after treatment were significantly changed(P<0.05),and the level of FBG and HDL had no significantly changed(P>0.05).Conclusion:YFFZBJ powder treated diabetes mellitus by improved the number of lactobacillus and Bifidobacterium,reduced the expression of LPS andIL-6,and improve blood sugar and blood fat metabolism.
作者
陈玲玲
张思为
李董平
陈伊莉
Chen Lingling;Zhang Siwei;Li Dongping(Chinese Medicine,Shenzhen People’s Hospital in Guangdong Province,Shenzhen 518020)
出处
《陕西中医》
2018年第4期415-418,共4页
Shaanxi Journal of Traditional Chinese Medicine
关键词
糖尿病/中西医结合疗法
@薏苡附子败酱散
动物实验
小鼠
Diabetes mellitus/integrated
Chinese traditional and western medicine therapy
@Yiyi Fuzi Baijiang Powder
Animal experimentation
Mice
