抗双链DNA抗体和抗核小体抗体定性检测对系统性红斑狼疮的诊断价值被引量：13

Values of qualitative assays for anti-ds-DNA and anti-nucleosome antibodies in diagnosis of systemic lupus erythematosus

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摘要目的:评价免疫印迹法抗双链DNA(ds-DNA)抗体、抗核小体抗体(ANuA)及绿蝇短膜虫间接免疫荧光法抗dsDNA抗体检测在系统性红斑狼疮(SLE)中的诊断价值。方法:分别运用免疫印迹法、绿蝇短膜虫间接免疫荧光法及ELISA法检测166例SLE疾病活动期(包括初发和复发)患者血清中抗ds-DNA抗体、ANuA的水平。以酶联免疫吸附法(ELISA)检测结果为标准,评价抗ds-DNA抗体和ANuA定性检测对SLE的诊断价值。结果:免疫印迹法检测抗ds-DNA抗体及ANuA诊断SLE的阳性符合率分别为73.33%、66.28%,绿蝇短膜虫间接免疫荧光法检测抗ds-DNA抗体诊断SLE的阳性符合率为68.57%;随着抗ds-DNA抗体和ANuA滴度的减小,其免疫印迹法或间接免疫荧光法检测阳性符合率均降低(P<0.05)。结论:抗ds-DNA抗体、ANuA定性检测诊断SLE的阳性率均不理想,建议在ELISA检测抗ds-DNA抗体和ANuA诊断SLE的基础上采用。 Objective:To assess the diagnostic value of immunoblot assay for anti-double stranded DNA(ds-DNA)antibodies and anti-nucleosome antibodies(ANuA),and crithidia luciliae based indirect immunofluorescence for anti-ds-DNA antibodies in systemic lupus erythematosus(SLE)patients.Methods:The levels of anti-ds-DNA antibodies and ANuA in sera of 166 patients in active stage of SLE(including initial and recurrent patients)were detected by immunoblotting,crithidia luciliae immunofluorescence and enzyme-linked immunosorbent assay(ELISA).The values of qualitative detections for anti-ds-DNA antibodies and ANuA in diagnosing SLE were evaluated taking ELISA as standard.Results:The positive coincident rate of immunoblotting for anti-ds-DNA antibodies and ANuA was 73.33%and 66.28%,respectively.The positive coincident rate of crithidia luciliae immunofluorescence test for anti-ds-DNA antibodies was 68.57%.The positive coincident rates of immunoblotting and indirect immunofluorescent assay were both decreased with titers of anti-ds-DNA antibodies and ANuA(P<0.05).Conclusions:The positive rates in diagnosis of SLE are not ideal using qualitative assays for ds-DNA antibody and ANuA,therefore,qualitative assay is recommended to be applied based on ELISA.

作者李娜孔宁邹和建万伟国 LI Na;KONG Ning;ZOU He-jian;WAN Wei-guo(Department of Rheumatology,Huashan Hospital,Fudan University,Shanghai 200040,China)

机构地区复旦大学附属华山医院风湿免疫科

出处《中国临床医学》 2018年第1期70-73,共4页 Chinese Journal of Clinical Medicine

基金国家自然科学基金(81302571) 上海市自然科学基金(13ZR1452100)~~

关键词系统性红斑狼疮抗双链DNA抗体抗核小体抗体免疫印迹法绿蝇短膜虫间接免疫荧光法 ELISA法 systemic lupus erythematosus anti-double stranded DNA antibody anti-nucleosome antibody immunoblot assay crithidia luciliae immunofluorescence test enzyme-linked immunosorbent assay

分类号 R593.2 [医药卫生—内科学]

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参考文献1

1姜莉,万伟国,苏媁,邹和建.抗核小体抗体检测在系统性红斑狼疮中的临床价值[J].临床内科杂志,2011,28(7):459-461. 被引量：1

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