摘要
建立了一种运用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定灵芝及其孢子粉中胆碱含量的方法。实验采用Agilent公司ZORBAX 300-SCX阳离子交换色谱柱(4.6 mm×250mm,5μm),柱温30℃,流动相为乙腈-乙酸铵溶液,等度洗脱,流量为1.0 mL/min,进样量为10μL;蒸发光散射检测器检测,漂移管温度为60℃,喷雾器为模式加热,雾化气为净化空气,气体压力为172.4 kPa。研究结果表明,该方法精密度、重复性、稳定性和回收率均良好,在6.25~200μg/mL范围内胆碱具有良好的线性关系,检出限为6μg/mL,定量限为12μg/mL,是一种快速高效检测灵芝及其孢子粉中胆碱的方法。
To establish an HPLC-ELSD method for determining the content of choline in Ganoderma lucidum spore powder.The experiment using Agilent company ZORBAX 300-SCX cation exchange chromatography column(4.6 mm×250 mm,5μm)as the stationary phase,the column temperature was set at 30℃,the mobile phase was 0.3%acetonitrile-ammonium acetate solution(60∶40),with isocratic elution,the flow rate was 1.0 mL/min,the injection volume was 10μL,the detector was evaporative light-scattering detector(ELSD),the drift tube temperature of the ELSD was set at 60℃,the spray mode was heated,and the purifying air pressure was 172.4 kPa.Research results show that the method precision,repeatability,stability and recovery rate are good.The choline showed a good linear relationship in the range of 6.25～200μg/mL.The limit of detection was 6μg/mL,and the limit of quantitation was 12μg/mL.The results show tant it is a fast and efficient analysis method to determinate the content of choline in Ganoderma lucidum spore powder.
作者
王亚涛
刘艳芳
张芝华
张忠
张劲松
王晨光
周帅
唐庆九
WANG Yatao;LIU Yanfang;ZHANG Zhihua;ZHANG Zhong;ZHANG Jingsong;WANG Chenguang;ZHOU Shuai;TANG Qingjiu(College of Food Science,Shanghai Ocean University,Shanghai 201306,China;National Engineering Research Center of Edible Fungi/Key Laboratory of Edible Fungi Resources and Utilization(Source),Ministry of Agriculture/Institute of Edible Fungi,Shanghai Academy of Agriculture Sciences,Shanghai 201403,China;School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China;Shanghai Pashun Biological Technology Co Ltd,Shanghai 201403)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2018年第1期33-37,共5页
Journal of Food Science and Biotechnology
基金
国家"十二五"科技支撑计划项目(2013BAD16B08-02)
