摘要
【目的】应用不同外植体建立蜈蚣草组织培养快速繁殖的技术体系,并进行组培苗对砷的富集研究。【方法】以蜈蚣草的孢子、幼芽,以及无菌苗的幼叶、幼茎和顶芽为外植体,以不同浓度的蜈蚣草混合匀浆配比不同浓度的植物激素,优选出蜈蚣草组培快繁的最适条件。【结果】幼叶为最佳外植体,其次是孢子,顶芽和茎最差。1/2MS+2,4-D 2 mg/L+蜈蚣草茎叶匀浆混合液(PVCLM)60 g/L为最佳的愈伤诱导培养基,愈伤诱导率可达100%;1/2MS+6-BA 2 mg/L+NAA 0.2 mg/L+PVCLM 200 g/L为最佳分化培养基,分化率达98.3%;1/2 MS+6-BA 2 mg/L+NAA 0.2 mg/L+PVCLM 200 g/L为最佳的丛生芽增殖培养基,增殖系数达1.1;1/4MS+NAA 0.2 mg/L为最佳生根培养基,生根率98%,以上涉及的培养基中琼脂浓度为7.8 g/L,蔗糖浓度为20 g/L,p H为5.8~6.0。组培苗移栽至尾矿库栽种,成熟后检测蜈蚣草中富集的砷的量达到3318 g/hm^2。【结论】摸索出一套适合工厂化的蜈蚣草组培快繁技术体系,组培苗对砷的富集量较野生型高。
【Objective】The objective of the present paper is to establish plant tissue culture and rapid propagation techniques using different explants of Pteris vittata Linn.And the arsenic enrichment of plantlet was analyzed.【Method】The spore,bud and young leaves,caulicle,apical buds of plantlet were used as explants,and the 1/2 MS+sucrose 20 g/L+phytagel 7.8 g/L(pH 5.8-6.0)were used as the basic medium,the phytohormone and homogenate of plant ratio testing were used to screen the medium formula for callus induction and subculture of P.vittata.【Result】The young leaves were the best explants,followed by spores,the caulicle and apical buds were the worst explants.The best medium formula for the callus induced from explants was 1/2MS+2,4-D 2 mg/L+PVCLM 60 g/L.The best differentiation medium formula for the seedling induced from callus was 1/2MS+6-BA 2 mg/L+NAA 0.2 mg/L+PVCLM 200 g/L.The best adventitious bud multiplication medium formula was 1/2MS+6-BA 2 mg/L+NAA 0.2 mg/L+PVCLM 200 g/L.In addition,the use of 1/4MS+NAA 0.2 mg/L could get a high rooting rate(98%).Therefore,using this tissue culture system,callus reduction and differentiation rate could reach to 100%and 98.3%.Finally,the concentration of arsenic enrichment of plantlet in P.vittata was 3318 g/hm 2 when planted in tailing impoundments.【Conclusion】A rapid tissue culture technology system was established for P.vittata.This technology is suitable for mass production.The plantlet can enrichment more arsenic than wild type.
作者
王玲仙
曾民
陈玲
付坚
柯学
王波
陈越
程在全
WANG Ling-xian;ZENG Min;CHEN Ling;FU Jian;KE Xue;WANG Bo;CHEN Yue;CHENG Zai-quan(Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650223,China)
出处
《西南农业学报》
CSCD
北大核心
2018年第3期623-629,共7页
Southwest China Journal of Agricultural Sciences
基金
云南省农产品产地土壤修复示范项目(2130139)
关键词
蜈蚣草
植株匀浆液
组培技术
砷富集
Pteris vittata Linn.
Plant homogenate
Tissue culture
Arsenic-accumulation
