摘要
目的依托哈尔滨医科大学大肠癌研究所,在东北地区建立一个规范化、标准化的结直肠肿瘤生物样本库。探究结直肠肿瘤组织样本的采集、保存,样本库构建方法,样本数据管理以及对所存储样本的质量控制技术,为结直肠肿瘤的发生、发展、转移、生物标志物的研究及转化医学和个体化治疗提供高质量的样本资源。方法收集自2013年于哈尔滨医科大学结直肠肿瘤外科诊断并行手术切除的结直肠肿瘤组织、癌旁组织、远端正常组织,将样本置于-80℃低温冰箱冷冻保存;同时定期对所取样本进行随机的DNA、RNA质量检测。结果截止2017年10月,本生物样本库共收集了440例患者的肿瘤、癌旁、正常组织及少量的血液样本共1 828份。且随机抽取样本的DNA、RNA质量检测结果均符合标本质量标准,能够满足科学实验需求。结论初步建立了一定规模标准化、规范化的结直肠肿瘤样本库,制定了标准化的样本库运行和管理规程,提高了生物样本利用的效率,为结直肠肿瘤基础和临床研究提供了宝贵的生物样本资源。
Objective Relying on the Institute of Colorectal Cancer of Harbin Medical University,a standardized biobank of colorectal cancer was established in the northeast of China.To explore the collection,preservation of colorectal tumor tissue samples,construction method of biobank,sample data management and quality control of stored samples,which can provide high quality samples of colorectal tumors occurrence,development,metastasis,biomarker research,translational medicine and individualized treatment.Methods The colorectal cancer tissues,para-carcinoma tissues and distal normal tissues were resected from colorectal cancer surgery at Harbin Medical University since 2013.The samples were stored at-80℃low temperature freezer for cryopreservation.At the same time,a random DNA and RNA quality test is carried out on the sampled samples.Results Up to October 2017,a total of 1 828 samples of tumor,para-carcinoma tissues,normal tissue and a small amount of blood samples were collected from 440 patients.In addition,the quality of DNA and RNA detected by random sampling are in accordance with the quality standards of samples,which can meet the needs of scientific experiments.Conclusion We have initially established a standardized colorectal tumor biobank,established standardized biobank operation and management procedures,improved the efficiency of biological sample utilization,and provided precious biological sample resources for basic and clinical research of colorectal tumors.
作者
于洋
王贵玉
Yu Yang;Wang Guiyu(Department of Colorectal Cancer Surgey,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China;2Colorectal Cancer Research Institute of Harbin Medical University,Harbin 150086,China)
出处
《中华结直肠疾病电子杂志》
2018年第2期167-171,共5页
Chinese Journal of Colorectal Diseases(Electronic Edition)
基金
中国教育部博士点新教授基金(No.20122307120025)
哈尔滨市科技创新人才研究专项资金(No.2012RFQXS054)
博士后研究人员落户黑龙江科研启动资助金(No.LBH-Q15174)
