摘要
目的利用ApoE基因缺陷小鼠建立动脉粥样硬化(AS)模型,研究AS进展过程中脂蛋白相关磷脂酶A2(Lp-PLA2)水平的变化及其在血管组织中的分布,并比较Lp-PLA2及其他生物标志物与AS的相关性。方法将已敲除Apo E基因的小鼠随机分为两组,对照组饲以一般饲料,实验组饲以高脂饮食,建立AS模型。定期取小鼠血清测定Lp-PLA2及其他血脂、炎症指标,并取主动脉染色做抗Lp-PLA2免疫组织化学染色。结果实验组Lp-PLA2明显高于对照组(P<0.05),Lp-PLA2与周龄呈显著的线性相关。多因素分析所得回归方程为Y=0.767778xLp-PLA2+0.000594xTG(r^2=0.89658),此方程可用于评估AS进展程度。Lp-PLA2随AS病程发展在斑块内表达逐渐增多,主要分布在脂质核中心以及巨噬细胞富集的区域;AS中后期Lp-PLA2大量分布在变薄的纤维帽下。结论与传统风险因子相比,Lp-PLA2与AS的相关性更好,与AS斑块不稳定性相关,可以用于评估AS进展程度。
Objective We established atherosclerosis(AS)animal model to study the tendency of lipoprotein-associated phospholipase A2(Lp-PLA2)variation,and distribution of Lp-PLA2 in vascular tissues.Methods ApoE-deficient mice were randomly divided into two groups,of which control group fed on chow diet,the experimental group fed on high fat diet.Mice were randomly picked up to determine serum Lp-PLA2 and other biomarkers,and the aortas were dissected to be immunohistochemically stained with anti-Lp-PLA2.Results Lp-PLA2 levels in the experimental group were significantly higher than those in control group(P<0.05).There was also significantly linear correlation between Lp-PLA2 and week-of-birth.Multiple factors analysis showed the equation was Y=0.767778xLp-PLA2+0.000594xTG(r2=0.89658),which could be used to estimate the progression of cardiovascular and cerebrovascular disease.The results from immunohistochemical staining indicated that early plaque had much less Lp-PLA2 than expanded plaques did.Lp-PLA2 mainly distributed in necrotic cores and around the macrophages.At later stage,a large number of Lp-PLA2 was located under the thinning fibrous cap.Conclusion Compared to traditional risk factors,Lp-PLA2 showes greater relevance to AS,and is also related to plaque vulnerability.
作者
黄立纲
刘炼华
刘航齐
贾玫
HUANG Li-gang;LIU Lian-hua;LIU Hang-qi;JIA Mei(Deparment of Laboratory Medicine,Peking University People′s Hospital,Beijing 100044,China)
出处
《中国医药生物技术》
2018年第2期151-156,共6页
Chinese Medicinal Biotechnology
