摘要
目的研究脱-γ-羧基凝血酶原(DCP)检测在早期肝细胞癌(HCC)临床诊断中的价值。方法选择2013~2016年本院收治的40例HCC患者;另外选择同期住院治疗的其他肝脏疾病患者40例作为肝病组,同期住院治疗的其他肿瘤患者作为肿瘤组,行健康体检的40例作为对照组,采用酶联免疫法及电化学发光法检测4组的DCP及甲胎蛋白(AFP),同时分析DCP和AFP浓度与肝癌临床特征的关联。结果 HCC组、肝病组、肿瘤组、对照组的DCP浓度(ng/ml)分别为642.70±467.80、8.96±6.71、10.62±7.71、3.69±2.00;AFP浓度(ng/ml)分别为781.00±683.90、8.92±7.65、9.10±8.42、4.16±2.83;其中HCC组的DCP浓度、AFP浓度均高于其他3组(P<0.01);肿瘤组、肝病组的平均DCP浓度与AFP浓度的差异无统计学意义(P>0.05)。根据HCC诊断界定值进行判定:DCP>12 ng/ml,灵敏度为80.0%,特异性为83.2%。AFP>20 ng/ml,灵敏度为75.0%,特异性为86.0%。两种方式联合检测的灵敏度为87.5%,特异性为88.7%。根据DCP与AFP浓度对病灶大小进行相关性分析:DCP浓度与患者的病灶直径的相关性有统计学意义(r=0.632,P<0.021)。结论DCP可作为检测HCC的重要手段,联合AFP阳性检出率更高,有利于患者的早期诊断。
Objective To observe the diagnostic value of des-gamma-carboxy prothrombin(DCP)and explore effective methods for early diagnosis of hepatocellular carcinoma(HCC).Methods Fourty cases of HCC patients were collected from 2013 to 2016 in the hospital;40 inpatients with other liver diseases and cancers except for HCC,and 40 healthy subjects were taken as control.ELISA and electrochemiluminescence immunoassay were used to detect DCP and AFP for comparison of DCP and AFP with those in the four groups so as to reveal the relations of their concentrations to HCC clinical characters.Results DCP concentrations(ng/ml)in HCC,liver tumors and liver disorders rather than HCC,and control groups were found to be 642.7±467.8,8.9 6±6.7 1,10.6 2±7.71 and 3.69±2.00 respectively;AFP concentrations(ng/m l)were 781.0±683.9,8.9 2±7.6 5,9.10±8.4 2 and 4.16±2.83 resectively.The average concentrations of DCP and AFP in HCC group were notably higher than those in the other three groups(P<0.01).No significant difference of the concentrations of DCP and AFP was seen between liver tumors and other liver disorders(P>0.05).Based on the cut-off value of DCP>1 2 ng/ml,the sensitivity of 80.0%and specificity of 83.2%were obtained while on the value of AFP>2 0 ng/ml,sesitivity of 75.0%and specificity of 86.0%were noted.A combined tests of DCP and AFP gave rise to the sensitivity and specificity of as high as 87.5%and 88.7%respectively.Correlation analysis showed that DCP concentration was positively related to tumor size(r=0.632,P<0.0 2 1)
作者
冯晓鸿
杜合娟
FENG Xiao-hong;DU He-juan(Departments of Laboratory Medicine,Gaoyou Hospital 225600)
出处
《临床输血与检验》
CAS
2018年第2期154-156,共3页
Journal of Clinical Transfusion and Laboratory Medicine
基金
南京医科大学科技发展面上项目(No.2013NJMU195)资助
关键词
脱-Γ-羧基凝血酶原
早期
肝细胞癌
诊断
Des-gamma-carboxy prothrombin
Early stage
Hepatocellular carcinoma
Diagnosis
