摘要
本研究从花生中克隆得到2个GPAT基因,分别命名为AhGPAT3和AhGPAT5。AhGPAT3基因全长为1653bp,编码550个氨基酸;AhGPAT5基因全长为1383bp,编码460个氨基酸,均属于GPATs蛋白质家族。通过实时定量PCR检测克隆到的GPAT基因在花生不同组织、种子不同发育时期、多种非生物逆境胁迫及多种激素处理下的表达模式。结果显示,AhGPAT3与高等植物的GPAT3和GPAT2亲缘关系最近;AhGPAT5与高等植物的GPAT5和GPAT7亲缘关系最近。AhGPAT3和AhGPAT5基因在种子发育初期和下胚轴中的表达量较高;另外,发现AhGPAT3基因很可能参与了花生对低温、高盐和干旱的抗性调节。
In this study,we found two GPAT genes,named as AhGPAT3 and AhGPAT5,which may encode GPAT proteins from the cDNA library of peanut by using the Bioedit software.The length of AhGPAT3 gene is 1653 bp,encoding 550 amino acids;and AhGPAT3 gene is 1383 bp,encoding 460 amino acids.The two genes belonged to the GPAT protein family.The expression of GPAT genes was detected by quantitative real-time PCR(qRT-PCR)in different tissues,through different developmental stages of seeds,under abiotic stresses and a variety of hormone treatments.AhGPAT3 is closely related to GPAT3 and GPAT2 of higher plants,AhGPAT5 is closely related to GPAT5 and GPAT7 of higher plants.The expression of AhGPAT3 and AhGPAT5 genes was higher in the early stage of seed development and hypocotyls.The results suggested that AhGPAT3 gene may be involved in abiotic stresses regulation of peanut.
作者
郝翠翠
梁成伟
石蕾
李昊远
陈明娜
潘丽娟
王通
王冕
禹山林
陈娜
迟晓元
HAO Cui-cui;LIANG Cheng-wei;SHI Lei;LI Hao-yuan;CHEN Ming-na;PAN Li-juan;WANG Tong;WANG Mian;YU Shan-lin;CHEN Na;CHI Xiao-yuan(College of Marine Science and Biological of Engineering,Qingdao University of Science&Technology,Qingdao 266042,China;Shandong Peanut Research Institute,Qingdao 266100,China;College of Marine and Technology,Harbin Institute of Technology,Weihai 264209,China)
出处
《花生学报》
北大核心
2018年第1期1-10,18,共11页
Journal of Peanut Science
基金
2014年国家"万人计划"青年拔尖人才(W02070268)
国家花生产业技术体系(CARS-13)
山东省自然科学基金(ZR2014YL012
ZR2014YL011)
国家自然科学基金(31000728
31100205
31200211)
山东省农业科学院青年英才培养计划
