摘要
以海生大型红藻多管藻(Polysiphonia urceolata)为材料,建立了从多管藻藻胆蛋白提取液中有效分离纯化藻蓝蛋白(R-PC)和别藻蓝蛋白(AP)的层析技术方法,即先使用Sephacryl S-300(S-300)和Sephacryl S-200(S-200)2种凝胶过滤层析从藻胆蛋白提取液中获得R-PC/AP组分,再依次经过Q Sepharose Fast Flow离子交换层析和Phenyl Sepharose 6 Fast Flow疏水层析对凝胶过滤获得的R-PC/AP组分进一步纯化.
An optimized technical procedure for simultaneous preparation of the R-phycocyanin(R-PC)and allophycocyanin(AP)from the extracted phycobiliprotein solution of marine red alga Polysiphonia urceolata is established based on the present experiments.R-PC and AP are isolated from the extracted phycobiliprotein solution of Polysiphonia urceolate using gel filtration through Sephacryl S-300(S-300)and S-200(S-200).The obtained fractions of R-PC and AP are further purther purified using ion exchange chromatography through Q Sepharose Fast Flow,then by using hydrophobic interaction chromatography through Phenyl Sepharose 6 Fast Flow.
作者
王胜楠
刁海平
赵明日
孙力
WANG Sheng-nan;DIAO Hai-ping;ZHAO Ming-ri;SUN Li(School of Life Sciences,Yantai University,Yantai 264000,China)
出处
《烟台大学学报（自然科学与工程版）》
CAS
2018年第2期105-113,共9页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
国家自然科学基金资助项目(40976083)
关键词
凝胶过滤
离子交换
疏水层析
多管藻
藻蓝蛋白
别藻蓝蛋白
gel filtration
ion exchange chromatography
hydrophobic interaction chromatography
Polysiphonia urceolata
R-phycocyanin
allophycocyanin
