摘要
目的从脑-肠轴角度分析胃肠疾病对睡眠的影响,为"胃不和则卧不安"理论提供现代科学支持。方法 SD大鼠随机分为正常组、模型组,针刺治疗组,药物治疗组,每组7只。采用束缚-浸水(restraint water-immersion stress,RWIS)应激法造大鼠胃溃疡模型;针刺治疗组选用"中脘""足三里",每天针刺20分钟,每5分钟行针30秒,药物组选用奥美拉唑肠溶片(0.2 mg/kg),每日一次灌胃给药,干预5天后取材,40 mg/kg戊巴比妥钠诱导大鼠睡眠,记录各组大鼠睡眠持续时间,Guth法计算胃黏膜损伤指数,和下酶联免疫法测定血清丘脑中胃泌素(gastrin,GAS)、生长抑素(somatostatin,SS)和褪黑素(melatonin,MT)的含量。结果模型组大鼠睡眠时间明显少于正常组(P<0.05),针刺治疗后睡眠时间明显延长(P<0.05);模型组大鼠胃黏膜损伤指数显著高于正常组(P<0.01),造胃溃疡模型成功;针刺组和药物组大鼠损伤指数较模型组明显降低(P<0.05)。模型组大鼠血清和下丘脑GAS较正常组含量均显著升高(P<0.01),MT、SS含量显著降低(P<0.01);针刺组和药物组大鼠血清和下丘脑GAS含量较模型组均明显降低(P<0.01),针刺组大鼠血清和下丘脑MT、SS的表达明显升高(P<0.01)。结论胃肠疾病与失眠密切相关,针刺"中脘""足三里"能够促进大鼠胃溃疡的愈合,改善大鼠睡眠,这与其降低外周和中枢GAS含量,增加SS、MT含量有关。
Objective To analysis the effects of gastrointestinal diseases on sleep from the angle of the“brain-gut axis”,to provide modern scientific support for the theory of“Inharmonious stomach leads to insomnia”.Methods SD rats were randomly divided into the normal group,the model group,the acupuncture treatment group and the drug treatment group,7 rats in each group.The gastric ulcer model was induced by restraint and water-immersion stress(RWIS).The acupuncture treatment group were acupuncture at“Zhongwan”,“Zusanli”,20 minutes a day,acupuncture of 30 seconds every 5 minutes.The rats in drug treatment group were treated with omeprazole enteric-coated tablets(0.2 mg/kg),gavage of medicine once a day.After 5 days of intervention,40 mg/kg sodium pentobarbital was used to induce sleep,sleep duration of rats were recorded.The gastric mucosal injury index was calculated by Guth,and the contents of gastrin(GAS),somatostatin(somatostatin,SS)and melatonin(melatonin,MT)in thalamus were detected by enzyme linked immunosorbent assays.Results The sleep time of the rats in the model group was significantly less than that in the normal group(P<0.05),and the sleep time was obviously prolonged after the acupuncture treatment(P<0.05).The gastric mucosal injury index in the model group was significantly higher than that of the normal group(P<0.01).The rats in the model group of serum and hypothalamic GAS were significantly increased compared with normal group(P<0.01),MT,SS content decreased significantly(P<0.01);the content of GAS in serum and hypothalamus of acupuncture treatment group and drug treatment group was significantly lower than that of model group(P<0.01).The expression of MT and SS in serum and hypothalamus of acupuncture treatment group increased significantly(P<0.01).Conclusion Gastrointestinal diseases and insomnia are closely related,acupuncture at“Zhongwan”and“Zusanli”can promote gastric ulcer healing,improve sleep in rats,which might contribute to decreasing the content of GAS and increasing the content of SS,MT in peripheral and central content.
作者
马佳佳
闫亚南
关翰宇
付晨露
李婧婷
薛婷
王旒靖
邵一轩
熊敏
葛东宇
董瑞娟
马惠芳
A Jiajia;YAN Yanan;GUAN Hanyu(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《环球中医药》
CAS
2018年第4期486-491,共6页
Global Traditional Chinese Medicine
关键词
应激性胃溃疡
针刺干预
脑肠轴
睡眠
胃泌素
生长抑素
褪黑素
Stress ulcer
Acupuncture intervention
Brain-Gut Axis
Sleep
Gastrin
Somatostatin
Melatonin
