摘要
目的观察槲皮素对2型糖尿病大鼠血糖的影响,并探讨其可能的机制。方法利用链脲佐菌素制备大鼠2型糖尿病模型。将SD大鼠随机分为对照组、模型组和槲皮素组,每组10只。取尾静脉血测各组大鼠的血糖,经心脏取血测定各组血清MDA含量和SOD活性,取肝脏测定各组丙酮酸激酶活性,取胰腺组织,以Western bloting法测定各组Bcl-2和Bax的表达情况。结果以高脂饲料喂养4周后,一次性腹腔注射链脲佐菌素可成功复制大鼠2型糖尿病模型,其血糖值高于对照组,差异具有统计学意义(P<0.05);与模型组相比,槲皮素组大鼠血糖水平明显降低,MDA含量降低,SOD活性升高,肝脏中丙酮酸激酶活性升高,胰腺组织中Bcl-2的表达水平显著增加,Bax的表达水平显著降低,差异均具有统计学意义(P<0.05)。结论槲皮素治疗能降低2型糖尿病大鼠的血糖,其机制可能与其降低机体氧化应激水平,抑制胰岛细胞凋亡及增强肝脏丙酮酸激酶活性有关。
Objective To observe the effect of quercetin on the blood glucose of type 2 diabetic rats,and investigate the potential mechanism.Methods The type 2 diabetic model of rat was made by intraperitoneal injecting streptozotocin at 45mg/kg body weight after 4 weeks high-fat diet.The Sprague Dawley rats were randomized into 3 groups,control group,model group and quercetin group(10 rats per group).The blood sugar level was measured by use of Glucose meter.The blood was taken from heart to test the MDA content and SOD activity in serum.The activity of pyruvate kinase was detected in liver.The Bcl-2 and Bax levels were determined in the rat’s pancreas by using of Western blotting.Results Intraperitoneal injection of streptozotocin after 4 weeks high-fat diet can successfully copy type 2 diabetic model of rat,and its blood glucose level was higher than normal,(P<0.05).The treatment with quercetin could reduce the blood glucose level of type 2 diabetic rats and the MDA content in serum,and increase the SOD activity increase and the activity of pyruvate kinase in liver(P<0.05).Meanwhile,compared with model group,quercetin could significantly increase the Bcl-2 level and decrease the Bax level in pancreas(P<0.05).Conclusion The treatment with quercetin can reduce the blood glucose level of type 2 diabetic rats,and the potential mechanism may associate with its role of attenuating the oxidative stress level,alleviating apoptosis of islet cells,and enhancing the pyruvate kinase activity.
作者
王建礼
杨作成
王聪
孙芳
徐兴华
WANG Jianli;YANG Zuocheng;WANG Cong;SUN Fang;XU Xinghua(School of Basic Medicine,Jining Medical University;Jining Medical University,Jining 272067,China)
出处
《济宁医学院学报》
2018年第2期135-138,共4页
Journal of Jining Medical University
基金
济宁医学院科研计划项目资助课题(JY2015KJ012)
