摘要
目的研究二烯丙基二硫(diallyl disulfide,DADS)对小鼠生精细胞的辐射防护效应。方法利用4Gy剂量X射线建立动物辐射损伤模型。观察辐照小鼠睾丸组织变化、精子活力及精子畸形率,检测蛋白质羰基含量、丙二醛(MDA)及8-羟基脱氧鸟苷(8-OHd G)含量,评估生精细胞的辐照损伤程度及DADS对生精细胞的防护效果。测定睾丸组织抗氧化酶系超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力及谷胱甘肽(GSH)含量,western blot检测Nrf2信号蛋白表达,探讨DADS的辐射防护机制。结果与单纯照射组相比,DADS预处理组睾丸组织损伤较轻,精子活力显著提高(P<0.05),精子畸形率降低(P<0.05),蛋白质羰基含量、MDA及8-OHd G水平明显下降(P<0.05)。SOD、CAT、GSH-Px及GSH等抗氧化指标显著提升(P<0.05),Nrf2表达明显增强。结论 DADS通过其抗氧化能力对雄性小鼠生精细胞急性辐射损伤具有一定的防护效应。
Objective To study the radiation-protective effect of diallyl disulfide(DADS)in germ cells of male mice.Methods Male mice were whole-body exposured to 4 Gy X-ray irradiation to establish a animal radiation damage model.Testicular histology,sperm motility and sperm deformity rate were observed,protein carbonyl content,malondialdehyde(MDA)and 8-hydroxy deoxyguanosine(8-OHdG)content were tested to assess the degrees of radiation damages of sperm cells and protective effect of DADS.Testicular tissue antioxidant enzymes such as superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-px)activity and glutathione(GSH)content were examined.The expression of Nrf2 signal protein was detected to explore the radiation-protective mechanism of DADS.Results Compared with the pure exposure group,the testicular tissue damages in the DADS pretreatment group were milder,sperm motility increased significantly(P<0.05)and sperm deformity rate decreased(P<0.05),and the protein carbonyl content,MDA and 8?OHdG levels significantly reduced(P<0.05).The antioxidant indexes of SOD,CAT,GSH-px and GSH were markedly improved(P<0.05),and the expression of Nrf2 was dramatically enhanced.Conclusions DADS has a protective effect on acute radiation injury in germ cells of male mice by means of anti-oxidative ability.
作者
许帅
李细霞
王敏
李署平
廖伟雄
XU Shuai;LI Xixia;WANG Min;LI Shuping;LIAO Weixiong(Zhaoqing Medical College,Zhaoqing 526020,China;The First People’s Hospital of Zhaoqing,Zhaoqing 526020)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第4期27-32,共6页
Chinese Journal of Comparative Medicine
基金
肇庆市科技创新指导类项目(20160403015)
肇庆医学高等专科学校中青年科技基金项目(Zqyq16-0010)
肇庆医学高等专科学校2015年度校级科研项目(2015K66)
