摘要
目的研究皮质酮慢性皮下注射对小鼠抑郁样行为伴发学习记忆损伤影响的研究。方法 40只雄性C57BL/6J小鼠,依据体重分成4组,空白组,皮质酮20、40、80 mg/kg组,通过皮下注射皮质酮21 d,采用空场实验、悬尾实验,检测小鼠抑郁样行为改变。并选用抑郁行为最明显的皮质酮剂量,通过悬尾和强迫游泳实验、物体位置识别实验、Morris水迷宫实验,研究皮质酮对小鼠抑郁伴发学习记忆损伤的影响。结果与空白组相比,皮质酮40、80 mg/kg剂量在空场实验的中央区(运动)路程、时间显著减少(P<0.05),皮质酮中剂量和高剂量在悬尾实验中能显著增长小鼠的不动时间(P<0.01或P<0.05)。强迫游泳实验显示,中剂量的皮质酮明显增加小鼠中的不动时间(P<0.05);物体认知实验结果显示,中剂量皮质酮可导致小鼠对物体位置识别实验的辨别指数降低;水迷宫实验结果显示,皮质酮中剂量给药组的水迷宫定位航行潜伏期比空白组明显增加(P<0.05),且皮质酮组在水迷宫空间探索实验中的平均速度、目标象限平均速度和目标象限穿台次数与空白组相比明显减少。结论皮质酮慢性注射可诱导小鼠抑郁样行为的改变并伴有学习记忆功能的损害。
Objective To Study the depression-like behavior and impairment of learning and memory induced by chronic injection of corticosterone in mice.Methods Forty male C57BL/6J mice were divided into four groups,the control group,and the corticosterone groups(20,40,80 mg/kg).The mice received subcutaneous injection of corticosterone once a day for 21 days.The depression-like behaviors were detected by open field test(OFT),tail suspension test(TST)and forced swimming test(FST).To select the most effective dose of corticosterone,TST,FST,object location recognition test,and Morris water maze(MWM)test were used to study the corticosterone-induced depression-like behaviors and impairment of learning and memory in the mice.Results Compared with the control group,the movement distance and duration were significantly decreased in the corticosterone injection groups(40,80 mg/kg)(P<0.01 or P<0.05).In the TST group,the immobilization time was significantly increased in the corticosterone injection group(40,80 mg/kg)(P<0.05).The TST and FST showed that the immobilization time of the corticosterone injection group(40 mg/kg)was significantly increased(P<0.05).The object recognition test showed that the discrimination indexes of the object location recognition were decreased in the corticosterone injection group(40 mg/kg).The MWM test showed that the escape latency was increased(P<0.05),and the number of crossing in target quadrant and the velocity in target quadrant were decreased(P<0.05)in the corticosterone injection group(40 mg/kg).Conclusions Chronic injection of corticosterone can induce depression accompanied with learning and memory impairment in mice.
作者
范琳犀
杨玉洁
姜宁
陈远伟
刘新民
王琼
FAN Linxi;YANG Yujie;JIANG Ning;CHEN Yuanwei;LIU Xinmin;WANG Qiong(The Sino.Portugal TCM International Cooperation Center,Affiliated TCM Hospital,Southwest Medical University,Luzhou 646000,China;Departent of Pharmacology,School of Pharmacy,Southwest Medical University,Luzhou 646000;Institute of Medicinal Plants Development,Academy of Medical Sciences and Peking Union Medical College,Beijing 100193;School of Pharmacy,Yunnan College of Traditional Chinese Medicine,Kunming 650500)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第4期59-64,共6页
Chinese Journal of Comparative Medicine
基金
中国医学科学院创新工程(2016-I2M-2-006)
国家重点研发计划(2016YFE0131800)
关键词
皮质酮
抑郁
学习记忆
corticosterone
depression-like behavior
learning and memory
mice
