摘要
为防控延边烟区烟草菌核病提供基础资料和理论依据,开展了病原菌鉴定及抗性种质材料筛选研究。将收集的病原菌核采用常规方法进行分离、纯化,经致病性测定、形态学特征观察、rDNA-ITS测序比对及基于rDNA-ITS序列的系统发育分析,鉴定病原菌种类;并采用离体叶片法、活体叶片法对61份种质材料进行抗性筛选。结果表明:(1)经形态学、分子生物学方法鉴定该病原菌为核盘菌[Sclerotinia sclerotiorum(Lib.)de Bary]。(2)两种方法筛选出31份高感材料、6份中感材料、15份抗病材料,鉴定结果一致率为85.25%,未筛选到免疫和高抗种质材料。延边烟区烟草菌核病病原菌为核盘菌,离体叶片法、活体叶片法可用于苗期烟草种质材料对菌核病抗性筛选。
Identification of pathogen and screening for resistant germplasm materials were conducted for providing fundamental data and theory for controlling tobacco sclerotinia rot in Yanbian tobacco areas.The sclerotium of pathogen were collected in 2015,and then isolated and purified by conventional methods.Pathogen was identified through pathogenicity test,morphological characteristics,sequence analysis of the internal transcribed spacer region of the ribosomal DNA(rDNA-ITS)and phylogenetic analysis based on rDNA-ITS sequences.61germplasm materials were screened by in vitro leaf-assay method and living leaf-assay method.The results showed that:(1)The pathogen was identified as[Sclerotinia sclerotiorum(Lib.)de Bary]by morphology and molecular biology methods.(2)31 high sensitive germplasms,6 medium sensitive germplasms and 15 resistance ones were identified by the two methods with a consistent rate of 85.25%.No immune or high resistant germplasms,was identified.The pathogen of tobacco sclerotinia rot was[Sclerotinia sclerotiorum(Lib.)de Bary]in Yanbian tobacco areas.In vitro leaf-assay and living leaf-assay methods can be used to screen the resistance of germplasm materials to sclerotinia rot at seedling stages of tobacco.
作者
高崇
吴国贺
李佰霖
安承荣
高歌农
卢宝慧
高玉亮
田慧敏
郑成叡
崔昌范
GAO Chong;WU Guohe;LI Bailin;AN Chengrong;GAO Genong;LU Baohui;GAO Yuliang;TIAN Huimin;ZHENG Chengrui;CUI Changfan(Yanbian Academy of Agricultural Sciences,Longjing Jilin 133400,China;Jilin Tobacco Monopoly Administration(Company),Changchun 130000,China;College of Agricultural,Jilin Agricultural University,Changchun 130118,China;College of life sciences,Chifeng university,Chifeng,Inner Mongolia 024000,China;Yanbian Branch of Jilin Tobacco Corporation Company,Yanji,Jilin 133000,China)
出处
《中国烟草科学》
CSCD
北大核心
2018年第2期69-75,共7页
Chinese Tobacco Science
基金
吉林省烟草专卖局(公司)项目"吉林省烟草有害生物调查研究"{[2010]83号}
关键词
烟草菌核病
离体叶片法
活体叶片法
抗性种质
tobacco sclerotinia rot
vitro leaf-assay method
living leaf-assay method
resistant germplasm
