摘要
[目的]本文旨在比较不结球白菜不同材料间叶片总花青苷含量及其抗氧化活性,探究花青苷合成相关基因的表达特性。[方法]以6种不结球白菜材料为试材,采用DPPH(1,1-二苯基-2-三硝基苯肼)法和铁离子还原能力(FRAP)法检测了叶片提取液抗氧化活性,并测定了花青苷合成相关结构基因(BrcF3H、BrcDFR、BrcANS、BrcCHI、BrcCHS、BrcUFGT)和调节基因(BrcTT8、BrcTTG1、BrcMYB75、BrcMYB90、BrcGL3、BrcEGL3.1、BrcEGL3.2)的表达水平。[结果]在6种不结球白菜材料中,深紫色材料NJZX1-4的总花青苷含量最高,为(801.5±57.4)μg·g^(-1)。6种材料叶片提取液的DPPH自由基清除率为(49.31±3.91)%^(84.94±6.20)%,铁离子还原能力为(256.8±136.3)^(1 279.5±202.0)μg·g^(-1),其中紫色材料NJZX1^(-1)叶片的DPPH自由基清除率和铁离子还原能力最强,且总花青苷含量与DPPH自由基清除率和铁离子还原能力均存在极显著正相关。结构基因BrcF3H、BrcDFR、BrcANS和调节基因BrcTT8在6种材料中的表达量与总花青苷的含量呈正相关。调节基因BrcMYB75和BrcTTG1在紫色材料NJZX1-4叶片中具有较高的表达量,而BrcEGL3.2和BrcMYB90基因在所有材料中均无表达。[结论]不结球白菜不同材料间花青苷含量和抗氧化活性存在显著差异,且紫色材料富含的花青苷在高抗氧化性方面发挥了重要作用。此外,3个结构基因和3个调节基因的表达水平与总花青苷含量密切相关,在调控不结球白菜叶片着色过程中起重要作用。
[Objectives]The aim of this study was to compare the antioxidant activity of total anthocyanins content in the leaves of different non-heading Chinese cabbage materials,and to investigate the expression patterns of genes related to anthocyanins biosynthesis in these materials.[Methods]We used six materials of non-heading Chinese cabbages,and measured the antioxidant activities of leaves by using DPPH(1,1-diphenyl-2-picrylhydrazyl)and FRAP(ferric reducing ability of plasma)methods.In addition,we determined the expression levels of related structural genes(BrcF 3 H,BrcDFR,BrcANS,BrcCHI,BrcCHS,BrcUFGT)and regulatory genes(BrcTT 8,BrcTTG1,BrcMYB75,BrcMYB90,BrcGL3,BrcEGL3.1,BrcEGL 3.2)related to anthocyanins biosynthesis.[Results]Among six materials,total anthocyanins content was the highest in the deep purple material NJZX1-4,which was(801.5±57.4)μg·g-1.DPPH free radical scavenging rates of six materials were(49.31±3.91)%-(84.94±6.20)%,and FRAP values were(256.8±136.3)-(1 279.5±202.0)μg·g-1,which was higher in purple material NJZX1-1 than those of other materials.The correlation among total anthocyanins content,DPPH radical scavenging rate and FRAP antioxidant capacity were extremely significant.Moreover,we found that the expression of anthocyanins structural genes(BrcF 3 H,BrcDFR and BrcANS)and regulatory genes(BrcTT 8)were positively correlated with the content of total anthocyanins.Regulatory genes BrcEGL 3.2 and BrcMYB 90 had no expression in all materials,and BrcMYB 75 and BrcTTG 1 were highly expressed in the deep purple leaf material NJZX1-4.[Conclusions]There were significant differences in total anthocyanins content and antioxidant activity among different non-heading Chinese cabbage materials,and anthocyanins played an important role in antioxidant activities in leaves.The expression levels of three anthocyanins structural genes and three regulatory genes had close relation with the accumulation of anthocyanins in leaves,indicating that these genes might play an important role in regulating the coloring process in leaves of non-heading Chinese cabbage.
作者
董慧杰
韩克
侯喜林
张志硕
胡春梅
DONG Huijie;HAN Ke;HOU Xilin;ZHANG Zhishuo;HU Chunmei(College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2018年第3期422-428,共7页
Journal of Nanjing Agricultural University
基金
江苏省农业科技自主创新项目[CX(15)1015]
江苏省科技支撑计划项目(BE2013429)
关键词
不结球白菜
花青苷
抗氧化活性
结构基因
调节基因
non-heading Chinese cabbage
anthocyanins
antioxidant activity
structural genes
regulatory genes
