摘要
目的观察β-榄香烯对链脲佐菌素(Streptozocin,STZ)导致增殖性糖尿病性视网膜病变大鼠视网膜中血管内皮生长因子(VEGF)水平的表达,探讨其对增殖性糖尿病性视网膜病变大鼠视网膜的可能作用机制。方法选取80只Wistar大鼠,随机分为正常对照组(NC组)、STZ诱导成功的糖尿病模型组(DC组)、低剂量β-榄香烯给药组(BL组)与高剂量给药组(BH),各20只。给药组分别在手术显微镜下玻璃体腔内注射不同计量的β-榄香烯、苏木精-伊红染色,观察大鼠视网膜组织的病理学改变,取各实验分组中的视网膜切片,对VEGF采用免疫组织化学染色法,检测视网膜组织中VEGF的表达。结果 HE染色结果显示,NC组视网膜各组织结构显现清晰的层次,DC组较NC组视网膜变薄,且结构破坏,神经节细胞数减少;BL组较DC组略有提高;BH组视网膜结构较BL组有好转。免疫组织化学染色后,检测的各实验组视网膜结果显示,DC组、NC组、BL组、BH组大鼠VEGF阳性细胞核数目分别为(125.4671±8.4561)、(10.1324±2.0334)、(75.5123±7.6507)、(21.5364±1.7861)个,各组间差异均有统计学意义(P<0.05)。结论β-榄香烯可能成为治疗增殖期糖尿病性视网膜病变的新作用靶点,同时可以改善晚期糖尿病性视网膜病变的发展,延缓视网膜组织的进一步恶化。
Objective To observe the effect ofβ-elemene on the expression of vascular endothelial growth factor(VEGF)in the retina of rats with proliferative diabetic retinopathy caused by streptozocin(STZ),to investigate its possible mechanism of action on retinopathy of rats with proliferative diabetic retinopathy.Methods A total of 80 Wistar rats were selected.They were randomly divided into normal control group(NC group),STZ-induced diabetes model group(DC group),low-doseβ-elemene group(BL group)and high-dose group(BH group),20 rats in each group.In the administration group,different doses ofβ-elemene and hematoxylin-eosin staining were injected into the vitreous cavity under the surgical microscope respectively.The pathological changes of rat retina tissues were observed.Retinal slices from each experiment group were collected.Immunohistochemical staining was applied to VEGF,and the expression of VEGF in retinal tissues was detected.Results The results of HE staining showed that the tissue structure of the retina in the NC group showed a clear level.The retina was thinner in DC group than that in the NC group,and the structure was broken.The number of ganglion cells was reduced;the BL group had a slight increase compared with the DC group.The retinal structure in the BH group was better than that in the BL group.After immunohistochemical staining,the retinal results in each experimental group showed that the number of VEGF-positive nuclei in the DC group,NC group,BL group and BH group was(125.4671±8.4561),(10.1324±2.0334),(75.5123±7.6507),(21.5364±1.7861)respectively,differences between the groups were statistically significant(P<0.05).Conclusionβ-elemene may become a new target for the treatment of proliferative diabetic retinopathy.At the same time,it can improve the development of advanced DR and delay the further deterioration of retinal tissue.
作者
帅天姣
代海燕
张召弟
陈丹丹
王瑞英
朴天华
SHUAI Tian-jiao;DAI Hai-yan;ZHANG Zhao-di;CHEN Dan-dan;WANG Rui-ying;PIAO Tian-hua(Department of Ophthalmology,Hongqi Hospital Affiliated to Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157000,China)
出处
《中国当代医药》
2018年第18期7-9,16,共4页
China Modern Medicine
基金
黑龙江省卫生计生委科研课题(2016-386)
