摘要
以一株分离自天津七里海湿地野生苇蘑为研究对象,以其菌丝体生物量为主要测量指标,对其菌丝体液体培养基进行优化,首先采用不同碳源、氮源进行单因素初选,再结合正交设计,最终得到其菌丝体最佳液体培养基配方为蔗糖20 g/L,蛋白胨4 g/L,Mg SO_4·7H_2O1.5 g/L,KH_2PO_43 g/L,V_(B1)0.004 g/L,p H值自然。29℃培养20 d,菌丝体生物量达到1.980 g/L。
A strain of wild weimo isolated from Tianjin Qilihai wetlands was studied in the experiment.The mycelial biomass was choosed as the main index to optimize liquid culture medium of wild weimo mycelium.Firstly,the single factor primary election was conducted with different carbon sources and nitrogen sources,then,in combination with orthogonal design.The optimum liquid nutrient medium was:20 g/L sucrose,4 g/L peptone,1.5 g/L MgSO·7H2O,3 g/L KH2PO4,0.004 g/L vitamin B1,pH natural.Under the condition of dark at 29℃,the biomass of mycelium could reach 1.980 g/L after culturing 20 days.
作者
徐营莉
郭成金
吕绍生
李兆江
刘西周
XU Ying-li;GUO Cheng-jin;Lü Shao-sheng(College of Life Sciences,Tianjin Normal University,Tianjin 300387;Tianjin Animal and Plant Resistance Key Laboratory,Tianjin 300387;Tianjin Qili Culture Communication Co.,Ltd.,Tianjin 301508)
出处
《安徽农业科学》
CAS
2018年第21期12-14,36,共4页
Journal of Anhui Agricultural Sciences
关键词
野生苇蘑
菌丝体
液体培养基
正交设计
生物量
Wild weimo
Mycelium
Liquid nutrient medium
Orthogonal design
Biomass
