摘要
[目的]比较黑棘鲷群体线粒体16S r RNA基因序列。[方法]运用黑棘鲷线粒体16Sr RNA基因片段,对来自我国沿海的10个野生黑棘鲷群体进行了遗传多样性以及遗传分化的分析。[结果]10个群体的80个个体中,碱基A、C、G、T的平均含量分别为31.6%、24.2%、22.7%、21.5%,一共定义了9种单倍型,共发现16个多态位点,得出9种单倍型,遗传距离为0.000 0~0.006 3,10个黑棘鲷群体的平均单倍型多样性(Hd)为0.393,平均核苷酸多样性指数(Pi)为0.003 19,说明10个群体表现出了较低的Hd和较低的Pi,其中厦门群体的遗传多样性最大,这与厦门的地理位置及气候有关。AMOVA模块和系统进化树均显示来自10个群体黑棘鲷间遗传分化不显著,说明来自南北方的黑棘鲷属于同一个管理单元,分子中性进化检验显示舟山群体显著偏离中性进化。[结论]该研究可为鲷科鱼类的进化以及遗传多样性研究提供依据。
[Objective]To compare 16S rRNA gene fragment of mitochondria from Acanthopagrus schlegelii.[Method]In this study,we used the 16S rRNA gene fragment of mitochondria from A.schlegelii to analyze the genetic diversity and genetic differentiation of 10 populations of wild A.schlegelii from the coast of China.[Result]The average content of bases A,C,G,T in 80 individuals in 10 groups was 31.6%,24.2%,22.7%,and 21.5%,respectively.A total of 9 haplotypes were defined,a total of 16 polymorphisms were found,and 9 haplotypes were obtained.The genetic distance was 0.000 0-0.006 3,the average Hd of 10 A.schlegelii groups was 0.393,and the average Pi was 0.003 19,indicating that 10 groups exhibit lower Hd and lower Pi,of which the Xiamen group had the largest genetic diversity.This was related to the geographical location and climate of Xiamen.Both the AMOVA module and the system evolution tree showed that the genetic differentiation between the A.schlegelii from 10 groups was not significant,indicating that the A.schlegelii from the North and South belonged to the same management unit,and the molecular neutral evolution test showed that the Zhoushan population was significantly deviated from the neutral evolution.[Conclusion]The study will provide evidence for the evolution and genetic diversity of A.schlegelii.
作者
王泽园
毛欣琪
上官静波
李贝贝
黎中宝
WANG Ze-yuan;MAO Xin-qi;SHANGGUAN Jing-bo(School of Fisheries,Jimei University,Xiamen,Fujian 361021;Fujian Key Laboratory of Marine Fishery Resources and Ecological Environment,Xiamen,Fujian 361021)
出处
《安徽农业科学》
CAS
2018年第21期122-126,132,共6页
Journal of Anhui Agricultural Sciences
基金
福建省自然科学基金项目(2017J01638)
集美大学创新团队基金项目(2010A004)
关键词
黑棘鲷
16SrRNA
遗传变异
Acanthopagrus schlegelii
16SrRNA
Genetic variation
