摘要
目的探讨IDO/TTS介导的色氨酸代谢途径在免疫性血小板减少症患者发病及治疗中的作用。方法选取2016年9月～2017年9月来我院就诊的30例免疫性血小板减少症患者(观察组)和同期来我院体检的30例健康者(对照组)为研究对象,观察组患者给予地塞米松进行治疗,采集治疗前后血液样本,比较分析血浆中Kyn、Trp、Kyn/Trp的水平,以及CD4^+、CD8^+T淋巴细胞中TTS、IDO的表达。结果治疗前,观察组Kyn、Trp以及Kyn/Trp水平均高于对照组,差异有统计学意义(P<0.05);治疗后,有效组患者血浆中Trp水平降低,Kyn、Kyn/Trp水平升高,差异有统计学意义(P<0.05);治疗前,观察组CD4^+、CD8^+T淋巴细胞中IDO水平显著高于对照组,TTS水平显著低于对照组,差异有统计学意义(P<0.05);治疗后,有效组患者CD4^+、CD8^+T淋巴细胞中IDO水平水平升高,TTS水平降低,差异有统计学意义(P<0.05);治疗无效组患者治疗前后各项指标无显著差异(P>0.05)。结论 IDO/TTS介导的色氨酸代谢途径与免疫性血小板减少症患者的发病相关,也可通过IDO/TTS评价患者的临床疗效,具有指导意义。
Objective To investigate the effect of IDO/TTS-mediated tryptophan metabolic pathway in the pathogenesis and treatment of immune thrombocytopenia.Methods 30 patients with immune thrombocytopenia(observation group)who came to our hospital from September 2016 to September 2017 and 30 healthy people(control group)who had physical examination in our hospital during the same period were selected as the study subjects.Patients in the observation group were treated with dexamethasone,and the blood samples before and after treatment were collected.The levels of Kyn,Trp,Kyn/Trp in the plasma,and the expressions of TTS and IDO in CD4+and CD8+T lymphocytes were analyzed and compared.Results Before treatment,the levels of Kyn,Trp and Kyn/Trp in the observation group were higher than those in the control group,and the difference was statistically significant(P<0.05);after treatment,the Trp level was decreased in plasma in the effective group.The levels of Kyn and Kyn/Trp were increased,and the difference was statistically significant(P<0.05);before treatment,IDO levels in CD4+and CD8+T lymphocytes in the observation group were significantly higher than those in the control group.The level of TTS was significantly lower than that in the control group,and the difference was statistically significant(P<0.05);after treatment,IDO levels in the CD4+and CD8+T lymphocytes in the effective group were increased,and the level of TTS was decreased.The differences were statistically significant(P<0.05);there was no significant difference in each index before and after treatment in the ineffective treatment group(P>0.05).Conclusion The IDO/TTS-mediated tryptophan metabolic pathway is associated with the onset of immune thrombocytopenia.IDO/TTS can also be used to evaluate the clinical efficacy of patients,with guiding significance.
作者
周珺
袁远
ZHOU Jun;YUAN Yuan(Clinical Laboratory,Taizhou Hospital in Zhejiang Province,Linhai 317000,China)
出处
《中国现代医生》
2018年第17期118-121,共4页
China Modern Doctor
基金
浙江省医药卫生科技计划项目(2015KYB442)
