摘要
目的:制备一种可生物降解有效安全的硫酸软骨素酶ABC(ChABC)和环磷酸腺苷(cAMP)缓释组织工程支架,使药物缓慢稳定释放,降低局部应用时对神经的刺激,促进中枢神经系统损伤后神经的修复和轴突的再生。方法:应用电纺丝技术制作的含ChABC及cAMP的聚碳酸亚丙酯及壳聚糖缓释组织工程支架,分析支架直径、载药量、包封率等参数,然后以磷酸盐缓冲液为体外释药介质观察组织工程支架的药物释放速度、药物的失活率及支架的降解速度。结果:ChABC和cAMP缓释组织工程支架在聚碳酸亚内酯质量浓度为8%、电压为10～15 kV、距离为15～20 cm时可以纺出纤维直径约3μm的平滑支架,单纯聚碳酸盐内酯纤维光滑,直径均一,壳聚糖微球光滑,聚碳酸亚内酯与壳聚糖混合后电纺丝形成的支架呈串珠样结构,其能缓慢持续释放有活性ChABC和cAMP,12 d后支架降解失重率约7%。结论:应用电纺丝方法成功制备含ChABC及cAMP的聚碳酸盐内酯及壳聚糖组织工程支架,其药物稳定释放,局部应用无神经刺激,可生物降解。
Objective:To prepare an effective and biodegradable tissue engineering scaffold with controlled release for Chondroitinase ABC(ChABC)and cyclic adenosine monophosphate(cAMP)which can release drug slowly and steadily with low nerve stimulation and promote central nerve system recovery and regeneration.Methods:ChABC-and cAMP-loaded polypropylene carbonate(PPC)and chitosan(CS)scaffold for tissue engineering was prepared by electrospinning.Analysis was performed on scaffold diameter,drug load,and encapsulation efficiency.Drug release rate and drug inactivation ratewas observed with phosphate buffer solution medium,and scaffold degradation rate was assessed.Results:ChABC and cAMP slow-release tissue engineering scaffolds in PPC possessed a mass concentration of 8%and voltage of 10~15 kV.At a length of 15~20 cm,it could be spun to a diameter of approximately 3μm.The pure PPC fiber was smooth and displayed a uniform diameter.The chitosan microsphere was smooth.The scaffold spun with a PPC and chitosan blend showed a beaded structure and could slowly release active ChABC and cAMP;12 days after construction,the scaffold showed a mass degradation rate of approximately 7%.Conclusions:Electrospinning method has been successfully applied to prepare PPC and CS tissue engineering scaffolds containing ChABC and cAMP;its advantages include stable drug release,local application without nerve stimulation,and biodegradability.
作者
姚军
田东
倪石磊
张付意
齐宏旭
YAO Jun;TIAN Dong;NI Shi-lei;ZHANG Fu-yi;QI Hong-xu(Department of Neurosurgery,Chengwu People's Hospital,Shandong 274200,China;Department of Neurosurgery,Qilu Hospital,Shandong University,Jinan 250012,China;The Laboratory of Advanced Materials and Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)
出处
《神经损伤与功能重建》
2018年第7期328-330,334,共4页
Neural Injury and Functional Reconstruction
基金
山东省医药卫生科技发展计划项目(No.2015WS0470)
关键词
硫酸软骨素酶ABC
环磷酸腺苷
电纺丝
聚碳酸亚丙酯
壳聚糖
chondroitinase ABC
cyclic adenosine monophosphate
electrospinning
polypropylene carbonate
chitosan
