摘要
激光诱导植物荧光寿命测量法是在植物荧光光谱分析法基础上开发的一种评估植物生长状况及环境监测的新技术。根据植物叶绿素荧光信号的物理特性,利用信息仿真技术开发了一种叶绿素荧光寿命校正方法,可提高植物叶绿素荧光寿命的测量精度。利用激光诱导叶绿素荧光寿命测量系统分别测得叶绿素荧光及其背景信号,先用解卷积法叶绿素荧光信号中分离出荧光衰减函数,可获取荧光寿命估计值。再结合叶绿素荧光寿命校正技术就能反演得到高精度的植物荧光寿命。仿真与实验结果表明:该方法可实现高精度的植物荧光寿命实时监测;并对不同含量的叶绿素提取液进行了测试,构建了植物荧光寿命与叶绿素含量的对应关系模型。未来该技术可用于遥感监测海洋、湖泊、河流中藻类植物的生物含量。
Laser-induced chlorophyll fluorescence lifetime measurement is a novel technique for evaluating plant growth status and environmental monitoring.This methodis developed on the basis of plant fluorescence spectrum analysis technique.According to the physical properties of chlorophyll fluorescence signal and information simulation technology,a laser-induced chlorophyll fluorescence lifetimecorrection technique for improving the chlorophyll fluorescence lifetime measurement accuracy is presented in this paper.Laser-induced plant fluorescence lifetime measurement system was used to collect the chlorophyll fluorescence and its background signal.Then,the chlorophyll fluorescence decay function can be separated from the plant fluorescence signal in deconvolution algorithm,and fluorescencelifetime estimation can be obtained.Finally,the chlorophyll fluorescence lifetime correction technique was used to retrieve the precision values of fluorescence lifetime.The results of simulation and experiment show that real-time monitoring of high-precision chlorophyll fluorescence lifetime can be achieved inthis method.Based on this,a large number of different concentrations chlorophyll solution were measured,and the relationship model of chlorophyll contentand the fluorescence lifetime was built.In the future,this method can be used for remote sensing to monitor the algae biomass in the ocean,lake or river.
作者
万文博
苏俊宏
WAN Wen-bo;SU Jun-hong(School of Optoelectronic Engineering,Xi’an Technological University,Xi’an 710021,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2018年第9期2835-2842,共8页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(61378050)
陕西省教育厅重点实验室科研计划项目(17JS051)
陕西省自然科学基础研究计划项目(2016JZ025)
西安工业大学光电工程学院院长基金项目(16GDYJZ03)资助
关键词
荧光寿命
激光诱导荧光
解卷积
校正技术
荧光雷达
Fluorescence lifetime
Laser-induced fluorescence
Deconvolution
Correction technique
Fluorescence LiDAR
