摘要
背景:大量研究已证实,骨髓间充质干细胞移植可促进损伤神经功能的恢复,但其来源有限,并且取材对人体创伤较大,因此寻找更加合适的种子细胞有很重要的意义。目的:观察胎盘间充质干细胞移植对阿尔茨海默模型大鼠行为学及脑内神经递质的改善作用。方法:将45只雄性SD大鼠随机分3组,正常组不进行任何干预或处理;模型组与实验组采用皮下注射D-半乳糖联合脑双侧海马注射β淀粉样肽25-35的方法,建立老年阿尔茨海默病模型,每组15只。造模成功后,向实验组大鼠双侧海马内注射胎盘间充质干细胞悬液(细胞浓度1×10~8 L^(-1),每侧5μL),模型组不进行细胞移植。细胞移植4周后,进行水迷宫行为学实验、脑组织匀浆神经递质检测及脑海马组织病理观察。结果与结论:(1)与正常组相比,模型组大鼠实验不同时间点的逃避潜伏期明显延长(P<0.05),在目标象限的活动路程减少(P<0.05);与模型组相比,实验组实验不同时间点的逃避潜伏期明显缩短(P<0.05),在目标象限的活动路程增多(P<0.05);(2)与正常组比较,模型组大鼠脑组织匀浆乙酰胆碱酯酶、单胺氧化酶水平显著升高(P<0.05),胆碱乙酰转移酶水平显著降低(P<0.05);与模型组比较,实验组大鼠脑组织匀浆乙酰胆碱酯酶、单胺氧化酶水平显著降低(P<0.05),胆碱乙酰转移酶水平显著升高(P<0.05);(3)与正常组比较,模型组神经数量明显减少,细胞间隙增大,胞体发生退化,细胞核出现固缩,核仁不明显,部分细胞出现空泡;与模型组比较,实验组神经形态较为完整,趋于正常,神经细胞数量有所增加;(4)结果显示,胎盘间充质干细胞移植可改善阿尔茨海默大鼠的学习记忆能力,调节其脑内神经递质水平。
BACKGROUND:A large number of studies have proved that bone marrow mesenchymal stem cell transplantation can promote the recovery of injured nerve function,but the cell source is limited.Moreover,cell collection is extremely traumatic to the human body,and it is very important to find more suitable seed cells.OBJECTIVE:To observe the effect of placental mesenchymal stem cell transplantation on behaviors and neurotransmitters in Alzheimer disease rats.METHODS:Forty-five male Sprague-Dawley rats were randomly divided into three groups(n=15 per group):normal group without any intervention or treatment,model group and experimental group treated with subcutaneous injection of D-galactose and bilateral hippocampal injection ofβ-amyloid peptide 25-35.The model of senile Alzheimer disease was established in the latter two groups.After the model was successfully established,placental mesenchymal stem cell suspension(1×108 cells/L,5μL)was injected into the hippocampus of the experimental group.The model group did not carry out cell transplantation.Four weeks after transplantation,Morris water maze for behavior observation,brain homogenate neurotransmitter detection and histopathological observation of the hippocampus were carried out.RESULTS AND CONCLUSION:(1)Compared with the normal group,the escape latency at different time points in the model group was significantly prolonged(P<0.05),and the activity path in the target quadrant was decreased(P<0.05).Compared with the model group,the escape latency at different time points in the experimental group was significantly shortened(P<0.05),and the activity path in the target quadrant was increased(P<0.05).(2)Compared with the normal group,the levels of monoamine oxidase and acetylcholinesterase in the brain homogenate increased significantly in the model group(P<0.05),while the level of choline acetyltransferase decreased significantly(P<0.05).Compared with the model group,the levels of acetylcholinesterase and monoamine oxidase in the brain homogenate of the experimental group decreased significantly(P<0.05),and the level of choline acetyltransferase increased significantly(P<0.05).(3)In the model group,the number of nerves decreased obviously,the cell space increased,the cell body degenerated,the nucleus became pyknosis,the nucleolus was not obvious,and vacuoles appeared in some cells.Compared with the model group,the nerve morphology of the experimental group was relatively complete and tended to be normal.The number of nerves in the experimental group was increased as compared with the model group.To conclude,placental mesenchymal stem cell transplantation can improve the learning and memory abilities of Alzheimer disease rats and regulate the level of neurotransmitters in the rat brain.
作者
何佳
鄢波
宋晓征
伍雪英
He Jia;Yan Bo;Song Xiao-zheng;Wu Xue-ying(Department of Neurology,363 Hospital,Chengdu 610041,Sichuan Province,China;Department of Neurology,West China Hospital of Sichuan University,Chengdu 610041,Sichuan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第29期4650-4656,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81201010)~~
