摘要
目的:研究槲皮素对人口腔角质细胞(human oral keratinocytes,HOKs)再生的生物学效应。方法:通过细胞计数试剂盒-8(cell counting kit-8,CCK-8)及迁移实验验证槲皮素对人牙龈角质细胞的增殖与迁移的药理作用。通过牙龈卟啉单胞菌脂多糖作为炎症刺激因子构建体外炎症模型,实时荧光定量聚合酶链反应(real time quantitative polymerase chain reaction,RT-qPCR)法检测转化生长因子(transforming growth factor,TGF)-β1和TGF-β3的表达及炎症因子白细胞介素(interleukin,IL)-1β和肿瘤坏死因子(tumor necrosis factor,TNF)-α的基因表达验证槲皮素的抗炎效果。酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测TGF-β1和TGF-β3的分泌水平。结果:20μmol/L槲皮素促进HOKs的增殖与迁移,并提高TGF-β3的mRNA水平与蛋白质表达,而50μmol/L槲皮素对TGF-β1的表达有促进作用。20、50μmol/L的槲皮素都有明显的抗炎作用。结论:20μmol/L槲皮素在体外实验中能够促进创面愈合并且促进HOKs再生。
Objective:To assess the potential of quercetin on gingival regeneration.Methods:Cell viability and proliferation capacity were tested by CCK-8 kit.Cell migration was monitored using Transwell chambers.An inflammatory situation was mimicked using Pg.LPS.RT-qPCR was performed to measure mRNA levels of IL-β1,TNF-α,TGF-β1,and TGF-β3.Protein levels of TGF-β1 and TGF-β3 were evaluated with ELISA.Results:20μmol/L quercetin enhanced the proliferation and migration of HOKs.Furthermore,20μmol/L quercetin increased both mRNA and protein levels of TGF-β3 under inflammatory conditions while 50μmol/L quercetin increased expression of TGF-β1.Expression of IL-β1 and TNF-αwere downregulated by quercetin treatment.Conclusion:Compared with untreated controls,the re-epithelialization of 20μmol/L quercetin-treated HOKs were highly rapid.
作者
米娅莎尔.呼加合买提
李雪
吕慧欣
丁鑫鑫
周延民
Miyashaer·Hujiahemaiti;LI Xue;LV Hui-xin;DING Xin-xin;ZHOU Yan-min(Department of Dental Implantology,School and Hospital of Stomatology,Jilin University,Changchun 130021,China)
出处
《口腔医学研究》
CAS
北大核心
2018年第9期991-994,共4页
Journal of Oral Science Research
关键词
槲皮素
角化龈
牙龈角质细胞
牙周软组织再生
Quercetin
Keratinized gingiva
Oral keratinocytes
Gingival re-epithelialization
