摘要
目的探讨小鼠受精时卵母细胞皮质颗粒胞吐的动态变化以及Synaptotagmin1(Syt1)对小鼠受精过程的影响。方法活细胞实时拍摄系统观察小鼠卵母细胞受精时皮质颗粒发生胞吐的动态变化特点。利用Syt1-Morpholino干扰方法,并设立对照,采用免疫荧光、Western blot和q RT-PCR方法分析Syt1在小鼠受精和不同时期受精卵中的作用,并统计分析Syt1对小鼠受精率的影响。结果活细胞实时拍摄系统成功显示了小鼠皮质颗粒胞吐的动态过程,未发现胞吐位置与纺锤体和染色体位置之间存在明确的位点关系,但是部分位置在卵母细胞极体附近。Syt1在小鼠受精后30 min^24 h不同时期受精卵中的表达量逐渐增加。Syt1表达降调后影响了小鼠受精及皮质颗粒分布,降低了小鼠受精率。结论用活细胞实时拍摄系统动态观察了小鼠皮质颗粒受精时胞吐的动态变化,Syt1直接参与了小鼠卵母细胞的受精过程。
Objective To investigate the dynamics of cortical granules exocytosis(CGE),and the role of Synaptotagmin1(Syt1)in mouse fertilization.Methods The dynamics of mouse CGE were filmed on Perkin Elmer precisely Ulta VIEW VOX confocal Imaging System.The Syt1 functions on mouse fertilization and expression in zygotes were analyzed by immunofluorescence,Western blot assy and qRT-PCR.Results The dynamic process of mouse CGE was observed after oocyte activation,and the exact pot seems to be close to the polar body.The Syt1 expression was gradually increased after fertilization.The mouse fertilization rate after Syt1 knockdown was significantly lower than that of the control group(P<0.05).Conclusion The dynamic process of CGE is studied.It is found that Syt1 is involved in mouse fertilization process.
作者
朱秀兰
徐丽清
阮建兴
唐婷
刘风华
ZHU Xiu-lan;XU Li-qing;RUAN Jian-xing;TANG Ting;LIU Feng-hua(Department of Reproductive Health and Infertility,Guangdong Women and Children Hospital,Guangzhou 510000,China)
出处
《天津医药》
CAS
北大核心
2018年第9期928-931,1035,共5页
Tianjin Medical Journal
基金
国家自然科学基金青年科学基金项目(81300479)
